Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/6568
Title: Trypanosoma cruzi Discret Typing Units (TcII and TcVI) in samples of patients from two municipalities of the Jequitinhonha Valley, MG, Brazil, using two molecular typing strategies.
Authors: Oliveira, Maykon Tavares de
Assis, Girley Francisco Machado de
Silva, Jaquelline Carla Valamiel de Oliveira e
Machado, Evandro Marques de Menezes
Silva, Glenda Nicioli da
Veloso, Vanja Maria
Macedo, Andréa Mara
Martins, Helen Rodrigues
Lana, Marta de
Keywords: Trypanosoma cruzi
Genotyping
Chronic patients
Issue Date: 2015
Citation: OLIVEIRA, M. T. de et al. Trypanosoma cruzi Discret Typing Units (TcII and TcVI) in samples of patients from two municipalities of the Jequitinhonha Valley, MG, Brazil, using two molecular typing strategies. Parasites & Vectors, v. 8, p. 568-579, 2015. Disponível em: <http://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-015-1161-2>. Acesso em: 16 jun. 2016.
Abstract: Background: Trypanosoma cruzi is classified into six discrete taxonomic units (DTUs). For this classification, different biological markers and classification criteria have been used. The objective was to identify the genetic profile of T. cruzi samples isolated from patients of two municipalities of Jequitinhonha Valley, MG, Brazil. Methods: Molecular characterization was performed using two different criteria for T. cruzi typing to characterize 63 T. cruzi samples isolated from chronic Chagas disease patients. The characterizations followed two distinct methodologies. Additionally, the RAPD technique was used to evaluate the existence of genetic intragroup variability. Results: The first methodology identified 89 % of the samples as TcII, but it was not possible to define the genetic identity of seven isolates. The results obtained with the second methodology corroborated the classification as TcII of the same samples and defined the classification of the other seven as TcVI. RAPD analysis showed lower intra-group variability in TcII. Conclusions: The results confirmed the preliminary data obtained in other municipalities of the Jequitinhonha Valley, showing a predominance of TcII, similar to that verified in northeast/south axis of Brazil and the first detection of TcVI in the study region. The second protocol was more simple and reliable to identify samples of hybrid character.
URI: http://www.repositorio.ufop.br/handle/123456789/6568
metadata.dc.identifier.doi: https://doi.org/10.1186/s13071-015-1161-2
ISSN: 1756-3305
metadata.dc.rights.license: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Fonte: o próprio artigo.
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