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Título: | Immunodiagnosis of canine visceral leishmaniasis using mimotope peptides selected from phage is played combinatorial libraries. |
Autor(es): | Machado, Christina Monerat Toledo Ávila, Ricardo Andrez Machado de NGuyen, Christophe Granier, Claude Bueno, Lilian Lacerda Carneiro, Cláudia Martins Souza, Daniel Menezes Carneiro, Rubens Antônio Chávez Orlórtegui, Carlos Fujiwara, Toshio |
Data do documento: | 2015 |
Referência: | MACHADO, C. M. T. et al. Immunodiagnosis of canine visceral leishmaniasis using mimotope peptides selected from phage is played combinatorial libraries. BioMed Research International, v. 2015, p. 1-10, 2015. Disponível em: <http://www.hindawi.com/journals/bmri/2015/401509/>. Acesso em: 15 out. 2015. |
Resumo: | ELISA and RIFI are currently used for serodiagnosis of canine visceral leishmaniasis (CVL). The accuracy of these tests is controversial in endemic areas where canine infections by Trypanosoma cruzi may occur.We evaluated the usefulness of synthetic peptides that were selected through phage display technique in the serodiagnosis of CVL. Peptides were chosen based on their ability to bind to IgGs purified from infected dogs pooled sera. We selected three phage clones that reacted only with those IgGs. Peptideswere synthesized, polymerizedwith glutaraldehyde, and used as antigens in ELISA assays. Each individual peptide or a mix of them was reactive with infected dogs serum. The assay was highly sensitive and specific when compared to soluble Leishmania antigen that showed cross-reactivity with anti-T. cruzi IgGs. Our results demonstrate that phage display technique is useful for selection of peptides that may represent valuable synthetic antigens for an improved serodiagnosis of CVL |
URI: | http://www.repositorio.ufop.br/handle/123456789/6274 |
DOI: | http://dx.doi.org/10.1155/2015/401509 |
ISSN: | 2314-6141 |
Licença: | This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Fonte: o próprio artigo. |
Aparece nas coleções: | DEACL - Artigos publicados em periódicos |
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Arquivo | Descrição | Tamanho | Formato | |
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ARTIGO_ImmunodiagnosisCanineVisceral.pdf | 1,34 MB | Adobe PDF | Visualizar/Abrir |
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