Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/6257
Title: Multi-antigen print immunoassay (MAPIA)-based evaluation of novel recombinant Leishmania infantum antigens for the serodiagnosis of canine visceral leishmaniasis.
Authors: Oliveira, Isaac Queiroz de
Silva, Rodrigo Araujo
Sucupira, Michel Vergne
Silva, Edmilson Domingos da
Reis, Alexandre Barbosa
Grimaldi Junior, Gabriel
Fraga, Deborah Bittencourt Mothé
Veras, Patrícia Sampaio Tavares
Issue Date: 2015
Citation: OLIVEIRA, I. Q. de et al. Multi-antigen print immunoassay (MAPIA)-based evaluation of novel recombinant Leishmania infantum antigens for the serodiagnosis of canine visceral leishmaniasis. Parasites & Vectors, v. 8, n. 45, p. 2-5, 2015. Disponível em: <http://www.parasitesandvectors.com/content/8/1/45>. Acesso em: 15 out. 2015.
Abstract: Background: Domestic dogs are the principal reservoir hosts of Leishmania infantum in regions where visceral leishmaniasis (VL) is endemic. Although serologic methods are frequently used for the screening of infected dogs, antibody-based tests require further assessment, due to lack of sensitivity and specificity. In this study, we employed a multi-antigen printing immunoassay (MAPIA) to compare the antibody responses to novel recombinant proteins of L. infantum with the potential for the detection of canine VL. Findings: MAPIA strips were prepared employing 12 recombinant proteins. Antibody reactivity to these antigens was compared using a panel of sera collected from clinically asymptomatic (n = 16) and symptomatic (n = 41) culture-positive animals. Our findings showed that the canine immune response to antigen differs between dogs and depends on infection status. Using this screening assay, when five out of the 12 antigens were combined, an overall 81% detection rate of L. infantum-infected dogs was achieved. Conclusions: We conclude that MAPIA is an effective screening tool to rapidly select multiple antigens of diagnostic utility to be used in a more sensitive point of care diagnostic test such as the Dual-Path Platform (DPP) multiplex test for the rapid detection of infected dogs.
URI: http://www.repositorio.ufop.br/handle/123456789/6257
metadata.dc.identifier.doi: https://doi.org/10.1186/s13071-015-0651-6
ISSN: 1756-3305
metadata.dc.rights.license: This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. Fonte: o próprio artigo.
Appears in Collections:DEACL - Artigos publicados em periódicos

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