Please use this identifier to cite or link to this item:
Title: Microbial dynamics during azo dye degradation in a UASB reactor supplied with yeast extract.
Authors: Silva, Silvana de Queiroz
Silva, Débora Cristiane
Lanna, Maria Célia da Silva
Baeta, Bruno Eduardo Lobo
Aquino, Sergio Francisco de
Keywords: Wastewater treatment
Microbial profile
Issue Date: 2014
Citation: SILVA, S. de Q. et al. Microbial dynamics during azo dye degradation in a UASB reactor supplied with yeast extract. Brazilian Journal of Microbiology, v. 45, p. 21-27, 2014. Disponível em: <>. Acesso em: 21 out. 2015
Abstract: The present work aimed to investigate the microbial dynamics during the anaerobic treatment of the azo dye blue HRFL in bench scale upflow anaerobic sludge bed (UASB) reactor operated at ambient temperature. Sludge samples were collected under distinct operational phases, when the reactor were stable (low variation of color removal), to assess the effect of glucose and yeast extract as source of carbon and redox mediators, respectively. Reactors performance was evaluated based on COD (chemical oxygen demand) and color removal. The microbial dynamics were investigated by PCR-DGGE (Polimerase Chain Reaction - Denaturing Gradient of Gel Electrophoresis) technique by comparing the 16S rDNA profiles among samples. The results suggest that the composition of microorganisms changed from the beginning to the end of the reactor operation, probably in response to the presence of azo dye and/or its degradation byproducts. Despite the highest efficiency of color removal was observed in the presence of 500 mg/L of yeast extract (up to 93%), there were no differences regarding the microbial profiles that could indicate a microbial selection by the yeast extract addition. On the other hand Methosarcina barkeri was detected only in the end of operation when the best efficiencies on color removal occurred. Nevertheless the biomass selection observed in the last stages of UASB operation is probably a result of the washout of the sludge in response of accumulation of aromatic amines which led to tolerant and very active biomass that contributed to high efficiencies on color removal.
ISSN: 1678-4405
metadata.dc.rights.license: Todo o conteúdo do periódico Brazilian Journal of Microbiology, exceto onde identificado, está sob uma licença Creative Commons que permite copiar, distribuir e transmitir o trabalho em qualquer suporte ou formato desde que sejam citados o autor e o licenciante. Não permite o uso para fins comerciais. Fonte: Brazilian Journal of Microbiology <>. Acesso em: 16 ago. 2019.
Appears in Collections:DEQUI - Artigos publicados em periódicos

Files in This Item:
File Description SizeFormat 
ARTIGO_MicrobialDynamicsDuring.pdf1,54 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.