Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/4916
Title: Choline oxidase chemilumin scent assay, after removal of serine from medium, of acetylcholine released in vitro from brain slices.
Authors: Prado, Marco Antônio Maximo
Santos, T. Moraes
Freitas, Renata Nascimento de
Silva, Marco Aurélio Romano
Gomez, Marcus Vinicius
Keywords: Acetylcholine assay
Chemiluminescent procedure
Neurotransmitter release
Issue Date: 1990
Citation: PRADO, M. A. M. et al. Choline oxidase chemilumin scent assay, after removal of serine from medium, of acetylcholine released in vitro from brain slices. Journal of Neuroscience Methods, v. 31, p. 193-196, 1990. Disponível em: <http://ac.els-cdn.com/0165027090901102/1-s2.0-0165027090901102-main.pdf?_tid=fcde7f20-a1a7-11e4-80c7-00000aab0f27&acdnat=1421870540_90c45cb48c2da9786322c32e22671e6b>. Acesso em: 08 nov. 2014.
Abstract: A chemiluminescent method has been used recently for the determination of acetylcholine with limitations such as the presence of a cholinesterase inhibitor in the incubation medium, which is indispensable for the study of acetylcholine release by various agents. A modified procedure is presented in which the cholinesterase inhibitor eserine (physostigmine) is extracted from the medium. The results showed complete recovery when labelled acetylcholine was used. This modified procedure was used to determine the release of acetylcholine evoked by tityustoxin and ouabain. The results were comparable to those obtained by bioassay using a strip of guinea pig ileum.
URI: http://www.repositorio.ufop.br/handle/123456789/4916
ISSN: 01650270
metadata.dc.rights.license: O Periódico Journal of Neuroscience Methods concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3556580424364.
Appears in Collections:DENCS - Artigos publicados em periódicos

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