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Título: | Abundance of tegument surface proteins in the human blood fluke Schistosoma mansoni determined by QconCAT proteomics. |
Autor(es): | Borges, William de Castro Simpson, Deborah M. Dowle, Adam A. Curwen, Rachel S. Oates, Jane Thomas Beynon, Robert J. Wilson, R. Alan |
Palavras-chave: | Schistosoma mansoni Quantitative proteomics |
Data do documento: | 2011 |
Referência: | BORGES, W. de C. et al. Abundance of tegument surface proteins in the human blood fluke Schistosoma mansoni determined by QconCAT proteomics. Journal of Proteomics, v. 74, p. 1519-1533, 2011. Disponível em: <http://www.sciencedirect.com/science/article/pii/S1874391911002685>. Acesso em: 08 nov. 2014. |
Resumo: | The schistosome tegument provides a major interface with the host blood stream in which it resides. Our recent proteomic studies have identified a range of proteins present in the complex tegument structure, and two models of protective immunity have implicated surface proteins as mediating antigens. We have used the QconCAT technique to evaluate the relative and absolute amounts of tegument proteins identified previously. A concatamer comprising R- or K-terminated peptides was generated with [13C6] lysine/arginine amino acids. Two tegument surface preparations were each spiked with the purified SmQconCAT as a standard, trypsin digested, and subjected to MALDI ToF-MS. The absolute amounts of protein in the biological samples were determined by comparing the areas under the pairs of peaks, separated by 6 m/z units, representing the light and heavy peptides derived from the biological sample and SmQconCAT, respectively. We report that aquaporin is the most abundant transmembrane protein, followed by two phosphohydrolases. Tetraspanin Tsp-2 and Annexin-2 are also abundant but transporters are scarce. Sm200 surface protein comprised the bulk of the GPI-anchored fraction and likely resides in the secreted membranocalyx. Two host IgGs were identified but in amounts much lower than their targets. The findings are interpreted in relation to the models of protective immunity. |
URI: | http://www.repositorio.ufop.br/handle/123456789/4777 |
DOI: | https://doi.org/10.1016/j.jprot.2011.06.011 |
ISSN: | 1874-3919 |
Licença: | O periódico Journal of Proteomics concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3550930717092. |
Aparece nas coleções: | DECBI - Artigos publicados em periódicos |
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Arquivo | Descrição | Tamanho | Formato | |
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ARTIGO_AbundanceTegumentSurface.pdf | 1,19 MB | Adobe PDF | Visualizar/Abrir |
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