Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/4520
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dc.contributor.authorSabioni, Mayra Xiomara Hernández-
dc.contributor.authorBaçante, Thales Augusto-
dc.contributor.authorVilela, Luciano-
dc.contributor.authorTafuri, Wagner Luiz-
dc.contributor.authorAfonso, Luís Carlos Crocco-
dc.contributor.authorVieira, Leda Quercia-
dc.date.accessioned2015-03-03T16:39:05Z-
dc.date.available2015-03-03T16:39:05Z-
dc.date.issued2006-
dc.identifier.citationSABIONI, M. X. H. et al. Vaccine-induced protection against Leishmania amazonensis is obtained in the absence of IL-12/23p40. Immunology Letters, v. 105, p. 38-47, 2006. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0165247805003883>. Acesso em: 08 nov. 2014.pt_BR
dc.identifier.issn0165-2478-
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/4520-
dc.description.abstractProtozoa of the genus Leishmania are intracellular parasites of macrophages and may cause diverse clinical forms of leishmaniasis, including cutaneous, diffuse cutaneous, mucocutaneous and visceral leishmaniasis. Infection with L. major in mice indicates that a protective immune response is achieved when Th1 cells are developed. Thus, adoptive or vaccine-induced protection against leishmaniasis is largely dependent on cell-mediated immunity and IFN-_ production. Induction of a Th1 response is dependent on the presence of IL-12 whilst lymphocytes are activated. This study was aimed at evaluating the role of IL-12 during infection with L. amazonensis and after vaccination with Leishvacin® (killed Leishmania amazonensis promastigotes), since the role of this cytokine in vaccine-induced immunity with this preparation in experimental models or in humans is not yet elucidated. Hence, C57BL/6 interleukin-12-deficient mice (IL-12p40−/−) and wild-type controls (wt) were infected with L. amazonensis and the course of infection, parasite burden and cytokine production were compared. IL-12p40−/− mice were more susceptible to L. amazonensis than wt: lesions and parasite burden were larger in IL-12p40−/− when compared to wt. Interestingly, IL-4 was not produced in the absence of IL-12 in response to infection with L. amazonensis. To evaluate the role of IL-12 in the vaccine-induced immunity against L. amazonensis infection, IL-12p40−/− wt mice were vaccinated in the base of the tail and subsequently challenged with L. amazonensis in the footpads. Surprisingly, vaccinated IL-12p40−/− mice developed smaller lesions and had fewer parasites in footpads than non-vaccinated controls. Lymph node and spleen cells from vaccinated IL-12p40−/− mice did not produce high levels of IFN-_ in response do in vitro stimulus with antigen. Hence, partial protection against infection with L. amazonensis could be obtained in the absence of functional IL-12 and a typical Th1 response.pt_BR
dc.language.isoen_USpt_BR
dc.subjectLeishmaniapt_BR
dc.subjectProtozoapt_BR
dc.subjectParasitept_BR
dc.subjectVaccinept_BR
dc.subjectAdjuvantpt_BR
dc.titleVaccine-induced protection against Leishmania amazonensis is obtained in the absence of IL-12/23p40.pt_BR
dc.typeArtigo publicado em periodicopt_BR
dc.rights.licenseO periódico Immunology Letters concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3530901376838.pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.imlet.2005.12.002-
Appears in Collections:DECBI - Artigos publicados em periódicos

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