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Title: Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats.
Authors: Gonçalves, Reggiani Vilela
Mezêncio, J. M. S.
Benevides, Gustavo Pereira
Mata, Sérgio Luis Pinto da
Neves, Clóvis Andrade
Sarandy, Mariáurea Matias
Vilela, Evaldo Ferreira
Keywords: Cicatrization
Healing ointment
Cutaneous wound
Issue Date: 2010
Citation: GONÇALVES, R. V. et al. Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats. Brazilian Journal of Medical and Biological Research, v. 43, p. 350-355, 2010. Disponível em: <>. Acesso em: 08 nov. 2014.
Abstract: This study determined the effects of gallium-aluminum-arsenide laser (GaAlAs), gallium-arsenide laser (GaAs) and Dersani® healing ointment on skin wounds in Wistar rats. The parameters analyzed were: type I and III collagen fiber concentrations as well as the rate of wound closure. Five wounds, 12 mm in diameter, were made on the animals’ backs. The depth of the surgical incision was controlled by removing the epithelial tissue until the dorsal muscular fascia was exposed. The animals were anesthetized with ketamine and xylazine via intraperitoneal injection. The rats were randomly divided into five groups of 6 animals each, according to the treatment received. Group 1 (L4): GaAs laser (4 J/cm2); group 2 (L30): GaAlAs laser (30 J/cm2); group 3 (L60): GaAlAs laser (60 J/cm2); group 4 (D): Dersani® ointment; group 5 (control): 0.9% saline. The applications were made daily over a period of 20 days. Tissue fragments were stained with picrosirius to distinguish type I collagen from type III collagen. The collagen fibers were photo-documented and analyzed using the Quantum software based on the primary color spectrum (red, yellow and blue). Significant results for wound closing rate were obtained for group 1 (L4), 7.37 mm/day. The highest concentration of type III collagen fibers was observed in group 2 (L30; 37.80 ± 7.10%), which differed from control (29.86 ± 5.15%) on the 20th day of treatment. The type I collagen fibers of group 1 (L4; 2.67 ± 2.23%) and group 2 (L30; 2.87 ± 2.40%) differed significantly from control (1.77 ± 2.97%) on the 20th day of the experiment.
ISSN: 1414-431X
metadata.dc.rights.license: No permission is required from the authors or the publishers download, reuse, reprint, modify, distribute, and/or copy articles published in the BJMBR, as long as the original authors and source are cited. Fonte: Brazilian Journal of Medical and Biological Research <>. Acesso em: 21 jan. 2014.
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