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http://www.repositorio.ufop.br/jspui/handle/123456789/4472
Título: | Enzyme immobilization on Ag nanoparticles/polyaniline nanocomposites. |
Autor(es): | Crespilho, Frank Nelson Iost, Rodrigo Michelin Travain, Silmar Antonio Oliveira Junior, Osvaldo Novais de Zucolotto, Valtencir |
Palavras-chave: | Silver nanocomposite Urease Polyaniline Enzyme immobilization Bioelectrochemistry |
Data do documento: | 2009 |
Referência: | CRESPILHO, F. N. et al. Enzyme immobilization on Ag nanoparticles/polyaniline nanocomposites. Biosensors & Bioelectronics, v. 24, n. 10, p. 3073-3077, jun. 2009. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0956566309001675>. Acesso em: 24 fev. 2015. |
Resumo: | The performance of the modified electrodes toward urea hydrolysis was investigated via amperometric measurements, revealing a fast increase in cathodic current with a well-defined peak upon addition of urea to the electrolytic solution. The cathodic currents for the ITO/PAni/PVA–AgNP/urease electrodes were significantly higher than for the ITO/PAni/urease electrodes. The friendly environment provided by the ITO/PAni/PVA–AgNP electrode to the immobilized enzyme promoted efficient catalytic conversion of urea into ammonium and bicarbonate ions. Using the Michaelis–Menten kinetics equation, a KM app of 2.7mmolL−1 was obtained, indicating that the electrode architecture employedmay be advantageous for fabrication of enzymatic devices with improved biocatalytic properties. |
URI: | http://www.repositorio.ufop.br/handle/123456789/4472 |
DOI: | https://doi.org/10.1016/j.bios.2009.03.026 |
ISSN: | 0956-5663 |
Licença: | O periódico Biosensors & Bioelectronics concede permissão para depósito do artigo no Repositório Institucional da UFOP. Número da licença: 3566051124860. |
Aparece nas coleções: | DEFIS - Artigos publicados em periódicos |
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ARTIGO_EnzymeImmobilizationNanoparticles.pdf | 770,69 kB | Adobe PDF | Visualizar/Abrir |
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