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Title: Enzyme immobilization on Ag nanoparticles/polyaniline nanocomposites.
Authors: Crespilho, Frank Nelson
Iost, Rodrigo Michelin
Travain, Silmar Antonio
Oliveira Junior, Osvaldo Novais de
Zucolotto, Valtencir
Keywords: Silver nanocomposite
Enzyme immobilization
Issue Date: 2009
Citation: CRESPILHO, F. N. et al. Enzyme immobilization on Ag nanoparticles/polyaniline nanocomposites. Biosensors & Bioelectronics, v. 24, n. 10, p. 3073-3077, jun. 2009. Disponível em: <>. Acesso em: 24 fev. 2015.
Abstract: The performance of the modified electrodes toward urea hydrolysis was investigated via amperometric measurements, revealing a fast increase in cathodic current with a well-defined peak upon addition of urea to the electrolytic solution. The cathodic currents for the ITO/PAni/PVA–AgNP/urease electrodes were significantly higher than for the ITO/PAni/urease electrodes. The friendly environment provided by the ITO/PAni/PVA–AgNP electrode to the immobilized enzyme promoted efficient catalytic conversion of urea into ammonium and bicarbonate ions. Using the Michaelis–Menten kinetics equation, a KM app of 2.7mmolL−1 was obtained, indicating that the electrode architecture employedmay be advantageous for fabrication of enzymatic devices with improved biocatalytic properties.
ISSN: 0956-5663
metadata.dc.rights.license: O periódico Biosensors & Bioelectronics concede permissão para depósito do artigo no Repositório Institucional da UFOP. Número da licença: 3566051124860.
Appears in Collections:DEFIS - Artigos publicados em periódicos

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