Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/4330
Title: Cytokine and nitric oxide patterns in dogs immunized with LBSap vaccine, before and after experimental challenge with Leishmania chagasi plus saliva of Lutzomyia longipalpis.
Authors: Resende, Lucilene Aparecida
Roatt, Bruno Mendes
Soares, Rodrigo Dian de Oliveira Aguiar
Viana, Kelvinson Fernandes
Mendonça, Ludmila Zanandreis de
Lanna, Mariana Ferreira
Lemos, Denise da Silveira
Oliveira, Rodrigo Corrêa de
Martins Filho, Olindo Assis
Fujiwara, Ricardo Toshio
Carneiro, Cláudia Martins
Reis, Alexandre Barbosa
Giunchetti, Rodolfo Cordeiro
Keywords: Vaccine
Canine visceral leishmaniasis
Cytokines
immunoprotection
Leishmania chagasi
Issue Date: 2013
Citation: RESENDE, L. A. et al. Cytokine and nitric oxide patterns in dogs immunized with LBSap vaccine, before and after experimental challenge with Leishmania chagasi plus saliva of Lutzomyia longipalpis. Veterinary Parasitology, v. 198, p. 371-381, 2013. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0304401713005050>. Acesso em: 13 ago. 2013.
Abstract: In the studies presented here, dogs were vaccinated against Leishmania (Leishmania) cha-gasi challenge infection using a preparation of Leishmania braziliensis promastigote proteinsand saponin as adjuvant (LBSap). Vaccination with LBSap induced a prominent type 1immune response that was characterized by increased levels of interleukin (IL-) 12 andinterferon gamma (IFN- _) production by peripheral blood mononuclear cells (PBMC) uponstimulation with soluble vaccine antigen. Importantly, results showed that this type ofresponsiveness was sustained after challenge infection; at day 90 and 885 after L. chagasichallenge infection, PBMCs from LBSap vaccinated dogs produced more IL-12, IFN- _ andconcomitant nitric oxide (NO) when stimulated with Leishmania antigens as comparedto PBMCs from respective control groups (saponin, LB- treated, or non-treated controldogs). Moreover, transforming growth factor (TGF)- _ decreased in the supernatant of SLcA-stimulated PBMCs in the LBSap group at 90 days. Bone marrow parasitological analysisrevealed decreased frequency of parasitism in the presence of vaccine antigen. It is con-cluded that vaccination of dogs with LBSap vaccine induced a long-lasting type 1 immuneresponse against L. chagasi challenge infection.
URI: http://www.repositorio.ufop.br/handle/123456789/4330
ISSN: 03044017
metadata.dc.rights.license: O Periódico Veterinary Parasitology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3440300508466.
Appears in Collections:DEACL - Artigos publicados em periódicos

Files in This Item:
File Description SizeFormat 
ARTIGO_CytokineNitricOxide.pdf573,88 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.