Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/4327
Title: Analysis using canine peripheral blood for establishing in vitro conditions for monocyte differentiation into macrophages for Leishmania chagasi infection and T-cell subset purification.
Authors: Viana, Kelvinson Fernandes
Soares, Rodrigo Dian de Oliveira Aguiar
Roatt, Bruno Mendes
Resende, Lucilene Aparecida
Lemos, Denise da Silveira
Oliveira, Rodrigo Corrêa de
Martins Filho, Olindo Assis
Moura, Sandra Aparecida Lima de
Zanini, Marcos Santos
Araújo, Márcio Sobreira Silva
Reis, Alexandre Barbosa
Giunchetti, Rodolfo Cordeiro
Keywords: Leishmania chagasi
Leishmaniasis visceral canine
Mcrobicidal analysis
Macrophage
Issue Date: 2013
Citation: VIANA, K. F. et al. Analysis using canine peripheral blood for establishing in vitro conditions for monocyte differentiation into macrophages for Leishmania chagasi infection and T-cell subset purification. Veterinary Parasitology, v. 198, p. 62-71, 2013. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0304401713004779>. Acesso em: 12 ago. 2014.
Abstract: Canine visceral leishmaniasis (CVL) is a parasitic disease endemic in many countries, anddogs present as the major natural reservoir of the parasite, Leishmania chagasi (syn. L.infantum). Biomarkers in the canine immune system is an important technique in thecourse of developing vaccines and treatment strategies against CVL. New methodologiesfor studying the immune response of dogs during Leishmania infection and after receivingvaccines and treatments against CVL would be useful. In this context, we used peripheralblood mononuclear cells (PBMCs) from healthy dogs to evaluate procedures related to (i)establishment of in vitro conditions of monocytes differentiated into macrophages infectedwith L. chagasi and (ii) purification procedures of T-cell subsets (CD4+and CD8+) usingmicrobeads. Our data demonstrated that after 5 days of differentiation, macrophages wereable to induce significant phagocytic and microbicidal activity after L. chagasi infectionand also showed increased frequency of parasitism and a higher parasite load. Although N-acetyl- _-d-glucosaminidase (NAG) levels presented similar levels of macrophage cultureand L. chagasi infection, a progressive decrease in myeloperoxidase (MPO) levels was ahallmark over 5 days of culture. High purity levels (>90%) of CD4 and CD8 T cells wereobtained on a magnetic separation column. We concluded that monocytes differentiatedinto macrophages at 5 days and displayed an intermediate frequency of parasitism andparasite load 72 h after L. chagasi infection. Furthermore, the purification system usingcanine T-lymphocyte subsets obtained after 5 days of monocyte differentiation provedefficient for CD4 or CD8 T-cell purification (≥90%). The in vitro analysis using L. chagasi-infected macrophages and purified T cells presented a prospective methodology that couldbe incorporated in CVL vaccine and treatment studies that aim to analyze the microbicidalpotential induced by specific CD4+and/or CD8+T cells.
URI: http://www.repositorio.ufop.br/handle/123456789/4327
metadata.dc.identifier.doi: https://doi.org/10.1016/j.vetpar.2013.08.014
ISSN: 0304-4017
metadata.dc.rights.license: O periódico Veterinary Parasitology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3440300468469.
Appears in Collections:DEACL - Artigos publicados em periódicos

Files in This Item:
File Description SizeFormat 
ARTIGO_AnalysisUsingCanine.pdf1,46 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.