Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/4318
Title: Parasite burden in hamsters infected with two different strains of Leishmania (Leishmania) infantum : ‘‘Leishman Donovan Units’’ versus real-time PCR.
Authors: Moreira, Nádia das Dores
Souza, Juliana Vitoriano de
Roatt, Bruno Mendes
Vieira, Paula Melo de Abreu
Ker, Henrique Gama
Cardoso, Jamille Mirelle de Oliveira
Giunchetti, Rodolfo Cordeiro
Lana, Marta de
Reis, Alexandre Barbosa
Keywords: Leishmania infantum
Polymerase chain reaction
Issue Date: 2012
Citation: MOREIRA, N. das D. et al. Parasite burden in hamsters infected with two different strains of Leishmania (Leishmania) infantum: ‘‘Leishman Donovan Units’’ versus real-time PCR. Plos One, v. 7, n. 10, p. e47907, 2012. Disponível em: <https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0047907>. Acesso em: 14 ago. 2014.
Abstract: To develop and test new therapeutics and immune prophylaxis strategies for visceral leishmaniasis (VL), understanding tissue parasitism evolution after experimental infection with Leishmania infantum is important. Experimental infection in a hamster model (Mesocricetus auratus) reproduces several typical aspects of canine and human VL that are closely related to the inoculum’s route. We quantified the parasitism in the liver and spleen of hamsters experimentally infected by various routes (intradermal, intraperitoneal, and intracardiac [IC]) and different strains of L. infantum (MHOM/BR/74/PP75 and Wild) and compared two different methodologies to evaluate tissue parasitism (Leishman Donovan units [LDU] and real-time qPCR). In addition, the quantification of specific total-IgG in the serum of uninfected and infected hamsters was determined by ELISA. The animals were followed for 1, 3, 6 and 9 months post-infection for survival analysis. We found that infection with the Wild strain by the IC route resulted in higher mortality. Positive antibody (IgG) responses were detected with higher peaks at 6 and 9 months in the IC group inoculated with PP75 strain. However, in animals infected with the Wild strain the IgG levels were elevated in all infected groups during all the time evaluated. We also observed by LDU analysis that the IC route lead to higher parasitism in the liver and spleen with both strains. Furthermore, qPCR showed higher sensitivity for identifying animals with low parasitic burden. In conclusion, qPCR can be useful for assessing parasitism in the spleen and liver of a hamster model infected with L. infantum independent of the route of infection, and this technique may become an essential tool for assessing parasite density in the hamster model after experimental treatment or immunization with potential vaccine candidates.
URI: http://www.repositorio.ufop.br/handle/123456789/4318
metadata.dc.identifier.doi: https://doi.org/10.1371/journal.pone.0047907
ISSN: 1932-6203
metadata.dc.rights.license: Os trabalhos publicados na Plos one estão sob Licença Creative Commons que permite copiar, distribuir e transmitir o trabalho, desde que sejam citados o autor e licenciante. Não permite o uso para fins comerciais nem a adaptação. Fonte: Plos one <https://www.plos.org/open-access>. Acesso em: 03 jan. 2017.
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