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Title: Evaluation of change in canine diagnosis Protocol adopted by the visceral Leishmaniasis control program in Brazil and a new proposal for diagnosis.
Authors: Vital, Wendel Coura
Ker, Henrique Gama
Roatt, Bruno Mendes
Soares, Rodrigo Dian de Oliveira Aguiar
Leal, Gleisiane Gomes de Almeida
Moreira, Nádia das Dores
Oliveira, Laser Antônio Machado de
Machado, Evandro Marques de Menezes
Morais, Maria Helena Franco
Oliveira, Rodrigo Corrêa de
Carneiro, Mariângela
Reis, Alexandre Barbosa
Keywords: Teste - ELISA
Canine visceral leishmaniasis
Issue Date: 2014
Citation: VITAL, W. C. et al. Evaluation of change in canine diagnosis Protocol adopted by the visceral Leishmaniasis control program in Brazil and a new proposal for diagnosis. Plos One, v. 9, n. 3, p. e91009, 2014. Disponível em: <>. Acesso em: 12 ago. 2014.
Abstract: The techniques used for diagnosis of canine visceral leishmaniasis (CVL) in Brazil ELISA and IFAT have been extensively questioned because of the accuracy of these tests. A recent change in the diagnosis protocol excluded IFAT and included the Dual-Path Platform (DPP). We evaluated the prevalence and incidence rates of Leishmania spp. before and after the change in the protocol. In addition, based on our results, we propose a new alternative that is less expensive for the screening and confirmation of CVL. Plasma samples were obtained from a serobank from dogs evaluated in a cross-sectional study (1,226 dogs) and in a cohort study of susceptible animals (n = 447), followed for 26 months. Serology testing wasperformed using ELISA, IFAT, and DPP. The incidence and prevalence of CVL were determined by using the protocol of the Visceral Leishmaniasis Control and Surveillance Program until 2012 (ELISA and IFAT using filter paper) and the protocol used after 2012 (DPP and ELISA using plasma). The prevalence was 6.2% and the incidence was 2.8 per 1,000 dog-months for the protocol used until 2012. For the new diagnosis protocol for CVL resulted in an incidence of 5.4 per 1,000 dog-months and a prevalence of 8.1%. Our results showed that the prevalence and incidence of infection were far greater than suggested by the previously used protocol and that the magnitude of infection in endemic areas has been underestimated. As tests are performed sequentially and euthanasia of dogs is carried out when the serological results are positive in both tests, the sequence does not affect the number of animals to be eliminated by the Control Program. Then we suggest to municipalities with a large demand of exams to use ELISA for screening and DPP for confirmation, since this allows easier performance and reduced cost.
ISSN: 1932-6203
metadata.dc.rights.license: Os trabalhos publicados na Plos one estão sob Licença Creative Commons que permite copiar, distribuir e transmitir o trabalho, desde que sejam citados o autor e licenciante. Não permite o uso para fins comerciais nem a adaptação. Fonte: Plos one <>. Acesso em: 03 jan. 2017.
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