Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/4308
Title: Basal and β-Adrenergic cardiomyocytes contractility dysfunction induced by dietary protein restriction is associated with downregulation of SERCA2a expression and disturbance of endoplasmic reticulum Ca2+ regulation in rats.
Authors: Penitente, Arlete Rita
Novaes, Rômulo Dias
Silva, Marcelo Eustáquio
Silva, Márcia Ferreira da
Quintão Júnior, Judson Fonseca
Guatimosim, Silvia
Cruz, Jader dos Santos
Chianca Júnior, Deoclécio Alves
Natali, Antônio José
Neves, Clóvis Andrade
Keywords: Intracellular calcium
Cardiovascular pathology
Isolated cardiomyocytes
Malnutrition - morphology
Issue Date: 2014
Citation: PENITENTE, A. R. et al. Basal and β-Adrenergic cardiomyocytes contractility dysfunction induced by dietary protein restriction is associated with downregulation of SERCA2a expression and disturbance of endoplasmic reticulum Ca2+ regulation in rats. Cellular Physiology and Biochemistry, v. 34, p. 443-454, 2014. Disponível em: <http://www.karger.com/Article/Pdf/363013>. Acesso em: 08 nov. 2014.
Abstract: Background: The mechanisms responsible for the cardiac dysfunction associated with dietary protein restriction (PR) are poorly understood. Thus, this study was designed to evaluate the effects of PR on calcium kinetics, basal and β-adrenergic contractility in murine ventricular cardiomyocytes. Methods: After breastfeeding male Fisher rats were distributed into a control group (CG, n = 20) and a protein-restricted group (PRG, n = 20), receiving isocaloric diets for 35 days containing 15% and 6% protein, respectively. Biometric and hemodynamic variables were measured. After euthanasia left ventricles (LV) were collected for histopathological evaluation, SERCA2a expression, cardiomyocytes contractility and Ca2+ sparks analysis. Results: PRG animals showed reduced general growth, increased heart rate and arterial pressure. These animals presented extracellular matrix expansion and disorganization, cardiomyocytes hypotrophy, reduced amplitudes of shortening and maximum velocity of contraction and relaxation at baseline and after β-adrenergic stimulation. Reduced SERCA2a expression as well as higher frequency and lower amplitude of Ca2+ sparks were observed in PRG cardiomyocytes. Conclusion: The observations reveal that protein restriction induces marked myocardial morphofunctional damage. The pathological changes of cardiomyocyte mechanics suggest the potential involvement of the β-adrenergic system, which is possibly associated with changes in SERCA2a expression and disturbances in Ca2+ intracellular kinetics.
URI: http://www.repositorio.ufop.br/handle/123456789/4308
ISSN: 14219778
metadata.dc.rights.license: This is an Open Access article licensed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported license (CC BY-NC) (), applicable to the online version of the article only. Distribution permitted for non-commercial purposes only. Fonte: Cell Physiol Biochem <http://www.karger.com/Article/FullText/363013.. Acesso em: 02 dez. 2014.
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