Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/4182
Title: Trypanosoma cruzi infection induces morphological reorganization of the myocardium parenchyma and stroma, and modifies the mechanical properties of atrial and ventricular cardiomyocytes in rats.
Authors: Novaes, Rômulo Dias
Penitente, Arlete Rita
Gonçalves, Reggiani Vilela
Silva, André Talvani Pedrosa da
Peluzio, Maria do Carmo Gouveia
Neves, Clóvis Andrade
Natali, Antônio José
Maldonado, Izabel Regina dos Santos Costa
Keywords: Cardiac myocytes
Chagas disease
Pathology
Extracellular matrix
Oxidative stress
Issue Date: 2013
Citation: NOVAES, R. D. et al. Trypanosoma cruzi infection induces morphological reorganization of the myocardium parenchyma and stroma, and modifies the mechanical properties of atrial and ventricular cardiomyocytes in rats. Cardiovascular Pathology, v. 22, p. 270-279, 2013. Disponível em: <http://www.sciencedirect.com/science/article/pii/S1054880712001536>. Acesso em: 08 nov. 2014.
Abstract: Background: This study investigates morphofunctional adaptations of the heart stroma and parenchyma in rats that are chronically infected with Trypanosoma cruzi. Methods: Four-month-old male Wistar rats were randomized into control (n=14) and infected (n=14) groups. Infected animals were inoculated with T. cruzi Y strain. After 9 weeks, the animals were euthanized, and the right atrium (RA) and left ventricle (LV) were removed for biochemical, stereological, and cardiomyocyte mechanical analyses. Results: Infected animals presented cardiomyocyte atrophy and myocardial fibrosis. For these animals, the total volume, length, surface area, and cross-sectional area of cardiomyocytes were significantly reduced, and the total interstitial and collagen volumes were significantly increased in the RA and LV compared to the controls. The total volume and length of blood vessels were significantly increased in the LV, and the total blood vessel surface area was significantly higher in the RA of infected animals. RA and LV cardiomyocytes from infected animals exhibited a significant reduction in cell shortening (43.02% and 24.98%, respectively), prolongation of the time to the peak of contraction (17.09%) and the time to half relaxation (23.68%) compared to non-infected animals. Lipid hydroperoxides, but not mineral concentrations, were significantly increased in the RA and LV from infected animals, showing an inverse correlation with cell shortening. Conclusions: T. cruzi infection induces global structural remodeling of the RA and LV in rats. This remodeling coexists with cardiomyocyte contractility dysfunction, which is possibly related to the abnormal organization of the myocardial stroma and increased cellular lipid peroxidation.
URI: http://www.repositorio.ufop.br/handle/123456789/4182
metadata.dc.identifier.doi: https://doi.org/10.1016/j.carpath.2012.12.001
ISSN: 1054-8807
metadata.dc.rights.license: O periódico Cardiovascular Pathology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3517120908487.
Appears in Collections:DECBI - Artigos publicados em periódicos

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