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Título: | Combination of extracts from Aristolochia cymbifera with streptomycin as a potential antibacterial drug. |
Autor(es): | Silva Júnior, Willer Ferreira da Cecílio, Samyra Giarola Magalhães, Cíntia Lopes de Brito Ferreira, Jaqueline Maria Siqueira Tótola, Antônio Helvécio Magalhães, José Carlos de |
Palavras-chave: | Aristolochia cymbifera Antibacterial activity Synergism Cytotoxicity Drug discovery |
Data do documento: | 2013 |
Referência: | SILVA JÚNIOR, W. F. et al. Combination of extracts from Aristolochia cymbifera with streptomycin as a potential antibacterial drug. SpringerPlus, v. 2, p. 430, 2013. Disponível em: <http://www.springerplus.com/content/pdf/2193-1801-2-430.pdf>. Acesso em: 08 nov. 2014. |
Resumo: | The appearance of new antibiotic-resistant bacteria is a societal problem that requires the development of new alternative treatments. Therefore, this work evaluated the antibacterial activity of ethanolic (EHI), dichloromethanic (EDI) and hexanic (EHE) extracts from Aristolochia cymbifera stems and the combination of these extracts with an antimicrobial drug to develop a new antibacterial therapy. The EDI, EHE and EHI extracts were obtained by maceration using three different solvents. The minimal inhibitory concentrations (MIC) of these extracts were determined using the microdilution test to determine the antibacterial potential of these extracts and their combination with streptomycin against Staphylococcus aureus, Bacillus cereus, Klebsiella pneumoniae and Shigella flexneri. The extract dose leading to the cytotoxicity of 50% of the cells (CC50) was evaluated using mammalian cells MA104 and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. The extracts had a MIC under 500 mg/L and a CC50 lower than 50 mg/L. The antibiotic/extract proportion influenced the antibacterial activity of the mixtures, and the proportion that optimized the antibacterial activity of streptomycin was a mixture that contained 75 percent of extract. This composition included less than 6.5 mg/L of extract and 2.5 mg/L of streptomycin and has potential as a new antibacterial therapy. |
URI: | http://www.repositorio.ufop.br/handle/123456789/4180 |
DOI: | https://doi.org/10.1186/2193-1801-2-430 |
ISSN: | 2193-1801 |
Licença: | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Fonte: SpringerPlus <http://www.springerplus.com/> Acesso em: 28 nov. 2014. |
Aparece nas coleções: | DECBI - Artigos publicados em periódicos |
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Arquivo | Descrição | Tamanho | Formato | |
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ARTIGO_CombinationExtractysAristolochia.pdf | 447,6 kB | Adobe PDF | Visualizar/Abrir |
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