Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/4027
Title: Usefulness of the polymerase chain reaction for monitoring cure of mice infected with different Trypanosoma cruzi clonal genotypes following treatment with benznidazole.
Authors: Miyamoto, Cláudia Tiemi
Gomes, Mônica Lúcia
Marangon, Aline Vansan
Araújo, Silvana Marques de
Bahia, Maria Terezinha
Martins Filho, Olindo Assis
Lana, Marta de
Toledo, Max Jean de Ornelas
Keywords: Trypanosoma cruzi
Benznidazole
Polymerase chain reaction
Issue Date: 2008
Citation: MIYAMOTO, C. T. et al. Usefulness of the polymerase chain reaction for monitoring cure of mice infected with different Trypanosoma cruzi clonal genotypes following treatment with benznidazole. Experimental Parasitology, v. 120, p. 45-49, 2008. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0014489408001215>. Acesso em: 28 ago. 2014.
Abstract: The capacity of the polymerase chain reaction (PCR) to detect the DNA of Trypanosoma cruzi was evaluated in 90 blood samples from BALB/c mice infected with T. cruzi cloned stocks of genotypes 19 and 20 (T. cruzi I) and 39 and 32 (T. cruzi II), and treated with benznidazole. The results from the fresh blood examination, hemoculture, and ELISA allowed to group the treated animals into: cured (TC), dissociated (DIS) and non-cured (NC). The PCR detected T. cruzi DNA in 50.9%, 58.3% and 100.0% of the samples from TC, DIS and NC mice, respectively. These DNA possibly derives from live T. cruzi or from recently lysed parasites, suggests that these animals are in fact not cured. The difference between the PCR results and results obtained using other techniques was statistically significant and independent of the parasite genotype. The PCR described has therefore potential to be used in cure control of treated patients.
URI: http://www.repositorio.ufop.br/handle/123456789/4027
metadata.dc.identifier.doi: https://doi.org/10.1016/j.exppara.2008.04.018
ISSN: 0014-4894
metadata.dc.rights.license: O periódico Experimental Parasitology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3462661422634.
Appears in Collections:DEACL - Artigos publicados em periódicos

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