Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/3821
Title: Comparison of polymerase chain reaction with other laboratory methods for the diagnosis of American cutaneous leishmaniasis Diagnosis of cutaneous leishmaniasis by polymerase chain reaction.
Authors: Marques, Marcos José
Volpini, Ângela Cristina
Coelho, George Luiz Lins Machado
Pinto, Jackson Machado
Costa, Carlos Alberto da
Mayrink, Wilson
Genaro, Odair
Romanha, Alvaro José
Keywords: American cutaneous leishmaniasis
Polymerase chain reaction
Diagnosis
Issue Date: 2006
Citation: MARQUES, M. J. et al. Comparison of polymerase chain reaction with other laboratory methods for the diagnosis of American cutaneous leishmaniasis Diagnosis of cutaneous leishmaniasis by polymerase chain reaction. Diagnostic Microbiology and Infectious Disease, v. 54, n.1, p. 37-43, 2006. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0732889305001926>. Acesso em: 01 set. 2014.
Abstract: An evaluation of 5 laboratory methods for diagnosing American cutaneous leishmaniasis (ACL) was carried out on patients from an endemic area of Brazil. From 164 patients presenting cutaneous lesions, and suspected to have ACL, 133 (81.1%) were confirmed for the disease by Montenegro skin test (MST) and/or parasitologic examination (PE). In both groups of patients, the positivity of polymerase chain reaction (PCR) was similar to that of immunofluorescence assay and enzyme-linked immunosorbent assay, and higher than that of MST and PE ( P b .05). In the group of patients suspected to have ACL, PCR presented the same positivity as PE and MST together. No correlation between positivity of the laboratory methods and clinical or epidemiologic aspects was observed. Our data confirmed the value of PCR as an alternative laboratory method for diagnosing ACL, especially for those patients with negative PE and MST.
URI: http://www.repositorio.ufop.br/handle/123456789/3821
metadata.dc.identifier.doi: https://doi.org/10.1016/j.diagmicrobio.2005.08.003
ISSN: 0732-8893
metadata.dc.rights.license: O periódico Diagnostic Microbiology and Infectious Disease concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3462040959725.
Appears in Collections:DEMSC - Artigos publicados em periódicos

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