Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/3771
Title: PLA-PEG nanocapsules radiolabeled with 99mTechnetium-HMPAO : release properties and physicochemical characterization by atomic force microscopy and photon correlation spectroscopy.
Authors: Pereira, Maira Alves
Mosqueira, Vanessa Carla Furtado
Vilela, José Mário Carneiro
Andrade, Margareth Spangler
Ramaldes, Gilson Andrade
Cardoso, Valbert Nascimento
Keywords: Nanocapsules
Atomic force microscopy
Physicochemical characterization
Photon correlation spectroscopy
Radiotracer
Issue Date: 2008
Citation: PEREIRA, M. A. et al. PLA-PEG nanocapsules radiolabeled with 99mTechnetium-HMPAO : release properties and physicochemical characterization by atomic force microscopy and photon correlation spectroscopy. European Journal of Pharmaceutical Sciences. V. 33, p. 42-51, 2008. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0928098707003636>. Acesso em: 20 ago. 2014.
Abstract: The present work describes the preparation, characterization and labelling of conventional and surface-modified nanocapsules (NC) with 99mTc-HMPAO. The size, size distribution and homogeneity were determined by photon correlation spectroscopy (PCS) and zeta potential by laser doppler anemometry. The morphology and the structural organization were evaluated by atomic force microscopy (AFM). The stability and release profile of the NC were determined in vitro in plasma. The results showed that the use of methylene blue induces significant increase in the encapsulation efficiency of 99mTc-HMPAO, from 24.4 to 49.8% in PLANC and 22.37 to 52.93% in the case of PLA-PEGNC(P < 0.05) by improving the complex stabilization. The average diameter of NC calculated by PCS varied from 216 to 323 nm, while the average diameter determined by AFM varied from 238 to 426 nm. The AFM analysis of diameter/height ratios suggested a greater homogeneity of the surface-modified PLAPEG nanocapsules compared to PLA NC concerning their flattening properties. The in vitro release of the 99mTc-HMPAO in plasma medium was faster for the conventional PLA NC than for the surface-modified NC. For the latter, 60% of the radioactivity remained associated with NC, even after 12h of incubation. The results suggest that the surface-modified 99mTc-HMPAO-PLA PEG NCwas more stable against label leakage in the presence of proteins and could present better performance as radiotracer in vivo.
URI: http://www.repositorio.ufop.br/handle/123456789/3771
ISSN: 0928-0987
metadata.dc.rights.license: O periódico Advanced Drug Delivery Reviews concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3460320800963.
Appears in Collections:DEFAR - Artigos publicados em periódicos

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