Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/3770
Title: HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies.
Authors: Oliveira, Liliam Teixeira
Garcia, Giani Martins
Kano, Eunice Kazue
Tedesco, Antônio Cláudio
Mosqueira, Vanessa Carla Furtado
Keywords: Chloroaluminium phthalocyanine
High-performance liquid
Nanocapsules
Photodynamic therapy
Fluorescent dye
Issue Date: 2011
Citation: OLIVEIRA, L. M. et al. HPLC-FLD methods to quantify chloroaluminum phthalocyanine in nanoparticles, plasma and tissue : application in pharmacokinetic and biodistribution studies. Journal of Pharmaceutical and Biomedical Analysis. V. 56, p. 70-77, 2011. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0731708511002275>. Acesso em: 20 ago. 2014.
Abstract: Analytical and bioanalytical methods of high-performance liquid chromatography with fluorescence detection (HPLC-FLD) were developed and validated for the determination of chloroaluminum phthalocyanine in different formulations of polymeric nanocapsules, plasma and livers of mice. Plasma and homogenized liver samples were extracted with ethyl acetate, and zinc phthalocyanine was used as internal standard. The results indicated that the methods were linear and selective for all matrices studied. Analysis of accuracy and precision showed adequate values, with variations lower than 10% in biological samples and lower than 2% in analytical samples. The recoveries were as high as 96% and 99% in the plasma and livers, respectively. The quantification limit of the analytical method was 1.12 ng/ml, and the limits of quantification of the bioanalytical method were 15 ng/ml and 75 ng/g for plasma and liver samples, respectively. The bioanalytical method developed was sensitive in the ranges of 15–100 ng/ml in plasma and 75–500 ng/g in liver samples and was applied to studies of biodistribution and pharmacokinetics of AlClPc.
URI: http://www.repositorio.ufop.br/handle/123456789/3770
metadata.dc.identifier.doi: https://doi.org/10.1016/j.jpba.2011.04.016
ISSN: 0731-7085
metadata.dc.rights.license: O periódico Journal of Pharmaceutical and Biomedical Analysis concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3457171409676.
Appears in Collections:DEFAR - Artigos publicados em periódicos

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