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dc.contributor.authorBrandão, Geraldo Célio-
dc.contributor.authorKroon, Erna Geessien-
dc.contributor.authorSouza, Danielle E. R.-
dc.contributor.authorSouza Filho, José Dias de-
dc.contributor.authorOliveira, Alaíde Braga de-
dc.identifier.citationBRANDÃO, G. C. et al. Chemistry and antiviral activity of Arrabidaea pulchra (Bignoniaceae). Molecules, v. 18, p. 9919-9932, 2013. Disponível em: <>. Acesso em: 20 ago. 2014.pt_BR
dc.description.abstractThe aim of the present work was to carry out a bioguided isolation of antiviral chemical constituents from an ethanol extract of leaves from Arrabidaea pulchra (Cham.) Sandwith (EEAPL) that had shown in vitro activity in a previous screening using DNA and RNA viruses. The activity of EEPAL was evaluated against the DNA viruses Human herpesvirus 1 (HSV-1) and Vaccinia virus Western Reserve (VACV-WR) as well as against the RNA viruses Murine encephalomyocarditis virus (EMCV), and Dengue virus 2 (DENV-2) by the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) colorimetric assay. Cytotoxicity was determined in LLCMK2 and Vero cells and the Selectivity Indexes (SI) were calculated. The most potent effect was observed against DENV-2 (EC50 46.8 ± 1.6 μg mL−1; SI 2.7). For HSV-1 and VACV-WR EC50 values > 200 μg mL−1 were determined, while no inhibition of the cytopathic effect was observed with EMCV. Bioguided fractionation of EEAPL by partition between immiscible solvents followed by chromatography over a Sephadex LH20 column afforded two arylpropanoid glycosides, verbascoside (AP 1) and caffeoylcalleryanin (AP 2), along with a terpenoid, ursolic acid (AP 3). AP 1 and AP 3 exhibited similar anti-DENV-2 profiles, with SI values of 3.8 and 3.1, respectively, while AP 2 was the most effective anti-DENV-2 constituent, with a SI of 20.0. Our results show that A. pulchra leaves ethanol extract (EEAPL) affords compounds with antiviral activity, mainly against DENV-2.pt_BR
dc.subjectAntiviral activitypt_BR
dc.subjectDengue viruspt_BR
dc.subjectArrabidaea pulchrapt_BR
dc.titleChemistry and antiviral activity of Arrabidaea pulchra (Bignoniaceae).pt_BR
dc.typeArtigo publicado em periodicopt_BR
dc.rights.licenseThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Fonte: Molecules <>. Acesso em: 08 set. 2014.pt_BR
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