Effect of inoculum composition on the microbial community involved in the anaerobic digestion of sugarcane bagasse.
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2023
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In anaerobic digestion (AD), the choice of inoculum type seems to be relevant for methane
production for complex substrates, such as lignocellulosic material. Previous work demonstrated
that the addition of fresh manure and ruminal fluid to anaerobic sludge improved methane
productivity and kinetics of AD of crude sugarcane bagasse (CSB). Considering that the
improvement of methane production could be a result of a more adapted microbial community,
the present study performed the Next Generation Sequencing analysis to identify changes in the
microbiome of anaerobic sludge inoculum, resulting from fresh manure and ruminal fluid
addition. In comparison with AD performed only with anaerobic sludge inoculum (50:50, U),
accumulated methane production was 15% higher with anaerobic sludge plus ruminal fluid
inoculum (50:50, UR) and even higher (68%) with anaerobic sludge with fresh bovine manure
inoculum (50:50, UFM), reaching the value of 143 NmLCH4.gVS
−1
. Clostridium species were highly
abundant in all inocula, playing an important role during the hydrolysis and fermentation of CSB,
and detoxifying potential inhibitors. Microbial composition also revealed the occurrence of
Pseudomonas and Anaerobaculum at UFM inoculum that seem to have contributed to the higher
methane production rate, mainly due to their hydrolytic and fermentative ability on
lignocellulosic substrates. On the other hand, the presence of Alcaligenes might have had a
negative effect on methane production due to their ability to perform methane oxidation.
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Lignocellulosic material, Anaerobic digestion, Methanogens, Fermentative bacteria, Hydrolytic bacteria
Citação
PEREIRA, A. R. et al. Effect of inoculum composition on the microbial community involved in the anaerobic digestion of sugarcane bagasse. Environmental Technology, 2023. Disponível em: <https://www.tandfonline.com/doi/full/10.1080/09593330.2023.2166877>. Acesso em: 01 ago. 2023.