Reduced-pressure alkaline pretreatment as an innovative and sustainable technology to extract protein from brewer’s spent grain.
Nenhuma Miniatura disponível
Data
2023
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
One of the bottlenecks related to the extraction of protein from lignocellulosic biomass is the development of an
efficient, selective, economical, and environmentally friendly process. This study investigated the reducedpressure alkaline pretreatment (RPAP) as an innovative approach for protein extraction from brewer’s spent
grain (BSG). Experiments were carried out at different temperatures (30–70 ◦C), pressures (150–1025 mBar),
times (1–7 h), and NaOH concentrations (20–180 mM) to obtain a high-yield and selective protein extraction
associated with low CO2 emissions and high processing capacity per batch. Protein concentration, total CO2
emissions per protein extracted, selectivity (related to sugars), and processing capacity were the responses
considered for optimization. The optimum condition (T = 70 ◦C; P = 455 mBar, t = 1 h, and [NaOH] = 180.00
mM) was able to extract 80% of the total protein present in BSG composition. This condition was also selective
(76.8%) and had a low carbon footprint (0.43 g CO2 g protein extracted − 1 ). Glutamic acid was the most abundant
amino acid in both raw BSG (23.4%) and protein hydrolysate (24.7%), followed by leucine (9.5% and 8.4%,
respectively), proline (8.4% and 11.8%, respectively), and aspartic acid (9.0% and 8.1%, respectively). RPAP was
demonstrated to be an efficient and sustainable technology for protein extraction from BSG. This opens up new
opportunities for BSG and other protein-rich lignocellulosic materials valorization in a biorefinery.
Descrição
Palavras-chave
Lignocellulosic biomass, Protein, Extraction, Amino acid, Carbon footprint
Citação
FONSECA, Y. A. da et al. Reduced-pressure alkaline pretreatment as an innovative and sustainable technology to extract protein from brewer’s spent grain. Journal of Cleaner Production, v. 416, artigo 137966, set. 2023. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0959652623021248>. Acesso em: 01 ago. 2023.