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Title: LBMPL vaccine therapy induces progressive organization of the spleen microarchitecture, improved Th1 adaptative immune response and control of parasitism in Leishmania infantum naturally infected dogs.
Authors: Roatt, Bruno Mendes
Cardoso, Jamille Mirelle de Oliveira
Reis, Levi Eduardo Soares
Moreira, Gabriel José Lucas
Gonçalves, Letícia Captein
Marques, Flávia de Souza
Moreira, Nádia das Dores
Vieira, Paula Melo de Abreu
Soares, Rodrigo Dian de Oliveira Aguiar
Giunchetti, Rodolfo Cordeiro
Reis, Alexandre Barbosa
Keywords: Canine visceral leishmaniasis
Leishmania infantum
Issue Date: 2022
Citation: ROATT, B. M. et al. LBMPL vaccine therapy induces progressive organization of the spleen microarchitecture, improved Th1 adaptative immune response and control of parasitism in leishmania infantum naturally infected dogs. Pathogens, v. 11, n. 9, artigo 974, 2022. Disponível em: <>. Acesso em: 01 ago. 2023.
Abstract: The spleen plays a central role in human and canine visceral leishmaniasis, where the activation of the immune response occurs in one of the tissues where Leishmania infantum reproduces. Therefore, this organ is both a target to understand the mechanisms involved in the parasite control and a parameter for assessing the therapeutic response. In this sense, this study aimed to evaluate the main histological, immunological and parasitological aspects in the spleen of symptomatic dogs naturally infected by L. infantum treated with the therapeutic vaccine LBMPL. For this, dogs were divided into four groups: dogs uninfected and untreated (NI group); L. infantum-infected dogs that were not treated (INT group); L. infantum-infected dogs that received treatment only with monophosphoryl lipid A adjuvant (MPL group); and L. infantum-infected dogs that received treatment with the vaccine composed by L. braziliensis promastigote proteins associated with MPL adjuvant (LBMPL group). Ninety days after the therapeutics protocol, the dogs were euthanized and the spleen was collected for the proposed evaluations. Our results demonstrated a reduction of hyperplasia of red pulp and follicular area of white pulp, increased mRNA expression of IFN-γ, TNF-α, IL-12 and iNOS, and decreased IL-10 and TGF-β1, and intense reduction of splenic parasitism in dogs treated with the LBMPL vaccine. These results possibly suggest that the pro-inflammatory environment promoted the progressive organization of the splenic architecture favoring the cellular activation, with consequent parasite control. Along with previously obtained data, our results propose the LBMPL vaccine as a possible treatment strategy for canine visceral leishmaniasis (CVL).
ISSN: 2076-0817
metadata.dc.rights.license: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// 4.0/). Fonte: PDF do artigo.
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