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dc.contributor.authorOstolin, Thais Lopes Valentim Di Paschoale-
dc.contributor.authorGusmão, Miriã Rodrigues-
dc.contributor.authorMathias, Fernando Augusto Siqueira-
dc.contributor.authorCardoso, Jamille Mirelle de Oliveira-
dc.contributor.authorRoatt, Bruno Mendes-
dc.contributor.authorSoares, Rodrigo Dian de Oliveira Aguiar-
dc.contributor.authorRuiz, Jeronimo Conceição-
dc.contributor.authorResende, Daniela de Melo-
dc.contributor.authorBrito, Rory Cristiane Fortes de-
dc.contributor.authorReis, Alexandre Barbosa-
dc.identifier.citationOSTOLIN, T. L. V. D. P. et al. A specific Leishmania infantum polyepitope vaccine triggers Th1-type immune response and protects against experimental visceral leishmaniasis. Cellular Immunology, v. 380, artigo 104592, out. 2022. Disponível em: <>. Acesso em: 01 ago. 2023.pt_BR
dc.description.abstractThe development of an immunogenic, effective, and safe vaccine is essential as an alternative for disease control. The present study aimed to evaluate the immunogenicity and efficacy potential of a polyepitope T-cell antigen candidate against visceral leishmaniasis in a murine model. BALB/c mice were immunized with three doses subcutaneously with Poly-T Leish alone or adjuvanted with Saponin plus Monophosphoryl lipid A, with 15-day intervals between doses, and challenged with 107 stationary-phase Leishmania infantum promastigotes via tail vein. Immunogenicity and parasitism in spleen and liver of immunized mice were evaluated 45 days postchallenge. Our results revealed that the immunization with Poly-T Leish and Poly-T Leish/SM increases the percentage of specific T (CD4+ and CD8+) lymphocytes proliferation in vitro after antigen-specific stimulation. Also, Poly-T Leish and Poly-T Leish/SM groups showed a high percentage of IFN-γ and TNF-α-producing T cells, meanwhile, the Poly-T Leish/SM group also showed an increased percentage of multifunctional T cells producing double and triple-positive (IFN-γ+TNF-α+IL-2+) cytokines. The immunization with Poly-T Leish or Poly-T Leish/ SM stimulated a decreased IL-4 and IL-10 compared to the Saline and adjuvant group. Poly-T Leish/SM immunized mice exhibit a noteworthy reduction in the parasite burden (spleen and liver) through real-time PCR (96%). Moreover, we observed higher nitrite secretion in 120-hour stimulated-culture supernatant using Griess method. We demonstrated that the Poly-T Leish/SM candidate was potentially immunogenic, providing enhancement of protective immune mechanisms, and conferred protection reducing parasitism. Our candidate was considered potential against visceral leishmaniasis, and eventually, could be tested in phase I and II clinical trials in dogs.pt_BR
dc.subjectVisceral leishmaniasispt_BR
dc.subjectLeishmania infantumpt_BR
dc.subjectProtozoan proteinspt_BR
dc.subjectPolyepitope vaccinept_BR
dc.titleA specific Leishmania infantum polyepitope vaccine triggers Th1-type immune response and protects against experimental visceral leishmaniasis.pt_BR
dc.typeArtigo publicado em periodicopt_BR
Appears in Collections:DEACL - Artigos publicados em periódicos

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