Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/jspui/handle/123456789/16566
Title: Calcitonin gene-related peptide exerts inhibitory effects on autophagy in the heart of mice.
Authors: Schavinski, Aline Zanatta
Machado, Juliano
Morgan, Henrique Jorge Novaes
Silva, Natalia Lautherbach Ennes da
Gomes, Sílvia de Paula
Kettelhut, Isis do Carmo
Navegantes, Luiz Carlos Carvalho
Keywords: Protein metabolism
Issue Date: 2021
Citation: SCHAVINSKI, A. Z. et al. Calcitonin gene-related peptide exerts inhibitory effects on autophagy in the heart of mice. Peptides, v. 22, artigo 170677, 2021. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0196978121001856>. Acesso em: 11 out. 2022.
Abstract: Calcitonin Gene-Related Peptide (CGRP) is a potent vasodilator peptide widely distributed in the central nervous system and various peripheral tissues, including cardiac muscle. However, its role in heart protein metabolism remains unknown. We examined the acute effects of CGRP on autophagy and the related signaling pathways in the heart mice and cultured neonatal cardiomyocytes. CGRP (100 μg kg− 1 ; s.c.) or 0.9 % saline was injected in awake male C57B16 mice, and the metabolic profile was determined up to 60 min. In fed mice, CGRP drastically increased glycemia and reduced insulinemia, an effect that was accompanied by reduced cardiac phosphoryla- tion levels of Akt at Ser473 without affecting FoxO. Despite these catabolic effects, CGRP acutely inhibited autophagy as estimated by the decrease in LC3II:LC3I and autophagic flux. In addition, the fasting-induced autophagic flux in mice hearts was entirely abrogated by one single injection of CGRP. In parallel, CGRP stimulated PKA/CREB and mTORC1 signaling and increased the phosphorylation of Unc51-like kinase-1 (ULK1), an essential protein in autophagy initiation. Similar effects were observed in cardiomyocytes, in which CGRP also inhibited autophagic flux and stimulated Akt and FoxO phosphorylation. These findings suggest that CGRP in vivo acutely suppresses autophagy in the heart of fed and fasted mice, most likely through the activation of PKA/ mTORC1 signaling but independent of Akt.
URI: http://www.repositorio.ufop.br/jspui/handle/123456789/16566
metadata.dc.identifier.doi: https://doi.org/10.1016/j.peptides.2021.170677
ISSN: 1873-5169
metadata.dc.rights.license: This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). Fonte: o PDF do artigo.
Appears in Collections:DECBI - Artigos publicados em periódicos

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