Different tidal volumes may jeopardize pulmonary redox and inflammatory status in healthy rats undergoing mechanical ventilation.
Nenhuma Miniatura disponível
Data
2021
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Mechanical ventilation (MV) is essential for the treatment of critical patients since it may provide a desired gas exchange. However,
MV itself can trigger ventilator-associated lung injury in patients. We hypothesized that the mechanisms of lung injury through redox
imbalance might also be associated with pulmonary inflammatory status, which has not been so far described. We tested it by
delivering different tidal volumes to normal lungs undergoing MV. Healthy Wistar rats were divided into spontaneously
breathing animals (control group, CG), and rats were submitted to MV (controlled ventilation mode) with tidal volumes of
4 mL/kg (MVG4), 8 mL/kg (MVG8), or 12 mL/kg (MVG12), zero end-expiratory pressure (ZEEP), and normoxia (FiO2 = 21%)
for 1 hour. After ventilation and euthanasia, arterial blood, bronchoalveolar lavage fluid (BALF), and lungs were collected for
subsequent analysis. MVG12 presented lower PaCO2 and bicarbonate content in the arterial blood than CG, MVG4, and
MVG8. Neutrophil influx in BALF and MPO activity in lung tissue homogenate were significantly higher in MVG12 than in
CG. The levels of CCL5, TNF-α, IL-1, and IL-6 in lung tissue homogenate were higher in MVG12 than in CG and MVG4. In
the lung parenchyma, the lipid peroxidation was more important in MVG12 than in CG, MVG4, and MVG8, while there was
more protein oxidation in MVG12 than in CG and MVG4. The stereological analysis confirmed the histological pulmonary
changes in MVG12. The association of controlled mode ventilation and high tidal volume, without PEEP and normoxia,
impaired pulmonary histoarchitecture and triggered redox imbalance and lung inflammation in healthy adult rats.
Descrição
Palavras-chave
Citação
CÂNDIDO, L. da S. et al. Different tidal volumes may jeopardize pulmonary redox and inflammatory status in healthy rats undergoing mechanical ventilation. Oxidative Medicine and Cellular Longevity, v. 2021, artigo 5196896, 2021. Disponível em: <https://link.springer.com/content/pdf/10.1007/s10532-022-09980-3.pdf?pdf=button>. Acesso em: 11 out. 2022.