Please use this identifier to cite or link to this item:
Title: Different tidal volumes may jeopardize pulmonary redox and inflammatory status in healthy rats undergoing mechanical ventilation.
Authors: Cândido, Leandro da Silva
Matos, Natália Alves de
Castro, Thalles de Freitas
Paul, Laisy Cristina de
Santos, Aline Maria dos
Costa, Guilherme de Paula
Silva, André Talvani Pedrosa da
Cangussú, Silvia Dantas
Zin, Walter Araújo
Bezerra, Frank Silva
Issue Date: 2021
Citation: CÂNDIDO, L. da S. et al. Different tidal volumes may jeopardize pulmonary redox and inflammatory status in healthy rats undergoing mechanical ventilation. Oxidative Medicine and Cellular Longevity, v. 2021, artigo 5196896, 2021. Disponível em: <>. Acesso em: 11 out. 2022.
Abstract: Mechanical ventilation (MV) is essential for the treatment of critical patients since it may provide a desired gas exchange. However, MV itself can trigger ventilator-associated lung injury in patients. We hypothesized that the mechanisms of lung injury through redox imbalance might also be associated with pulmonary inflammatory status, which has not been so far described. We tested it by delivering different tidal volumes to normal lungs undergoing MV. Healthy Wistar rats were divided into spontaneously breathing animals (control group, CG), and rats were submitted to MV (controlled ventilation mode) with tidal volumes of 4 mL/kg (MVG4), 8 mL/kg (MVG8), or 12 mL/kg (MVG12), zero end-expiratory pressure (ZEEP), and normoxia (FiO2 = 21%) for 1 hour. After ventilation and euthanasia, arterial blood, bronchoalveolar lavage fluid (BALF), and lungs were collected for subsequent analysis. MVG12 presented lower PaCO2 and bicarbonate content in the arterial blood than CG, MVG4, and MVG8. Neutrophil influx in BALF and MPO activity in lung tissue homogenate were significantly higher in MVG12 than in CG. The levels of CCL5, TNF-α, IL-1, and IL-6 in lung tissue homogenate were higher in MVG12 than in CG and MVG4. In the lung parenchyma, the lipid peroxidation was more important in MVG12 than in CG, MVG4, and MVG8, while there was more protein oxidation in MVG12 than in CG and MVG4. The stereological analysis confirmed the histological pulmonary changes in MVG12. The association of controlled mode ventilation and high tidal volume, without PEEP and normoxia, impaired pulmonary histoarchitecture and triggered redox imbalance and lung inflammation in healthy adult rats.
ISSN: 1942-0994
metadata.dc.rights.license: This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Fonte: o PDF do artigo.
Appears in Collections:DECBI - Artigos publicados em periódicos

Files in This Item:
File Description SizeFormat 
ARTIGO_DifferentTidalVolumes.pdf758,98 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.