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Title: Effects in vitro and in vivo of hesperidin administration in an experimental model of acute lung inflammation.
Authors: Souza, Ana Beatriz Farias de
Matos, Natália Alves de
Castro, Thalles de Freitas
Costa, Guilherme de Paula
Oliveira, Laser Antônio Machado de
Nogueira, Katiane de Oliveira Pinto Coelho
Ribeiro, Iara Mariana Léllis
Silva, André Talvani Pedrosa da
Cangussú, Silvia Dantas
Menezes, Rodrigo Cunha Alvim de
Bezerra, Frank Silva
Keywords: Antioxidants
Mechanical ventilation
Redox imbalance
Issue Date: 2022
Citation: SOUZA, A. B. F. de et al. Effects in vitro and in vivo of hesperidin administration in an experimental model of acute lung inflammation. Antioxidants, v. 180, p. 253-262, fev. 2022. Disponível em: <>. Acesso em: 11 out. 2022.
Abstract: Mechanical ventilation (MV) is a tool used in critical patient care. However, it can trigger inflammatory and oxidative processes capable of causing or aggravating lung injuries, which is known as ventilator-induced lung injury (VILI). Hesperidin is a flavonoid with antioxidant and anti-inflammatory properties in various diseases. The role of hesperidin in the process triggered by MV is poorly studied. Thus, we hypothesize hesperidin could protect the lung of mice submitted to mechanical ventilation. For that, we evaluated cell viability and reactive oxygen species (ROS) formation in macrophages using different hesperidin concentrations. We observed hes- peridin did not reduce cell viability, however; it attenuated the production of intracellular ROS in cells stimu- lated with lipopolysaccharide (LPS). We further evaluated the effects of hesperidin in vivo in animals submitted to MV. In the bronchoalveolar lavage fluid, there were higher levels of macrophage, lymphocyte and neutrophil counts in animals submitted to MV, indicating an inflammatory process. In the lung tissue, MV induced oxidative damage and increased myeloperoxidase activity, though the antioxidant enzyme activity decreased. MV also induced the production of the inflammatory mediators CCL-2, TNF-α and IL-12. Pretreatment with hesperidin resulted in less recruitment of inflammatory cells to the airways and less oxidative damage. Also, it reduced the formation of CCL-2 and IL-12. Our results show pretreatment with hesperidin can protect the lungs of mice submitted to mechanical ventilation by modulating the inflammatory response and redox imbalance and may act to prevent MV injury.
ISSN: 0891-5849
Appears in Collections:DECBI - Artigos publicados em periódicos

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