The exogenous surfactant pre-treatment attenuates ventilator-induced lung injury in adult rats.

Abstract

Mechanical ventilation is an essential supportive therapy in the treatment of critical patients, and it aims to maintain adequate gas exchange; however, it can also contribute to inflammation and oxidative stress, thus leading to lung injury. We tested the hypothesis that exogenous surfactant administration will be protective

against ventilator-induced lung injury in adult healthy Wistar rats both because of its anti-inflammatory prop- erties as well as its role in preventing alveolar collapse at end-expiration. Thus, the effect of intranasal instillation

of a bovine exogenous surfactant was tested in Wistar rats submitted to mechanical ventilation. The animals were

divided into four groups: (1) CONTROL; (2) SURFACTANT; (3) Mechanical ventilation (MV); (4) MV with pre- treatment with surfactant (MVSURFACTANT). The MV and MVSURFACTANT were submitted to MV with high

tidal volume (12 mL/kg) for 1 h. After the experimental protocol, all animals were euthanized and the arterial blood, bronchoalveolar lavage fluid and lungs were collected for biochemical, immunoenzymatic assay, arterial blood gases, and morphometric analyzes. The Wistar rats that received exogenous surfactant (Survanta®) by intranasal instillation before MV demonstrated reduced levels of leukocytes, inflammatory biomarkers such as CCL2, IL-1, IL-6 and TNF-α. Furthermore, it prevented oxidative damage by reducing lipid peroxidation and protein carbonylation as well as histological pattern changes of pulmonary parenchyma. Our data indicate that exogenous surfactant attenuated lung inflammation and redox imbalance induced by mechanical ventilation in healthy adult rats suggesting a preventive effect on ventilator-induced lung injury.