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Título : | Analgesic and side effects of intravenous recombinant Phα1β. |
Autor : | Rigo, Flavia Karine Rossato, Mateus Fortes Borges, Vanessa Silva, Juliana Figueira da Pereira, Elizete Maria Rita Ávila, Ricardo Andrez Machado de Trevisan, Gabriela Astoni, Duana Carvalho dos Santos Diniz, Danuza Montijo Silva, Marco Aurélio Romano Castro Junior, Célio José de Cunha, Thiago Mattar Ferreira, Juliano Gomez, Marcus Vinicius |
Palabras clave : | Analgesia Neuropathic pain Intravenous drug delivery system Cardiac function Motor activity |
Fecha de publicación : | 2020 |
Citación : | RIGO, F. K. et al. Analgesic and side effects of intravenous recombinant Phα1β. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 26, 2020. Disponível em: <https://www.scielo.br/j/jvatitd/a/mvYsWBBBDZDL8kfkXYm9PMk/?lang=en>. Acesso em: 11 out. 2022. |
Resumen : | Background: Intrathecal injection of voltage-sensitive calcium channel blocker peptide toxins exerts analgesic effect in several animal models of pain. Upon intrathecal administration, recombinant Phα1β exerts the same analgesic effects as the those of the native toxin. However, from a clinical perspective, the intrathecal administration limits the use of anesthetic drugs in patients. Therefore, this study aimed to investigate the possible antinociceptive effect of intravenous recombinant Phα1β in rat models of neuropathic pain, as well as its side effects on motor, cardiac (heart rate and blood pressure), and biochemical parameters. Methods: Male Wistar rats and male Balb-C mice were used in this study. Giotto Biotech® synthesized the recombinant version of Phα1β using Escherichia coli expression. In rats, neuropathic pain was induced by chronic constriction of the sciatic nerve and paclitaxel-induced acute and chronic pain. Mechanical sensitivity was evaluated using von Frey filaments. A radiotelemeter transmitter (TA11PA-C10; Data Sciences, St. Paul, MN, USA) was placed on the left carotid of mice for investigation of cardiovascular side effects. Locomotor activity data were evaluated using the open-field paradigm, and serum CKMB, TGO, TGP, LDH, lactate, creatinine, and urea levels were examined. Results: Intravenous administration of recombinant Phα1β toxin induced analgesia for up to 4 h, with ED50 of 0.02 (0.01-0.03) mg/kg, and reached the maximal effect (Emax = 100% antinociception) at a dose of 0.2 mg/kg. No significant changes were observed in any of the evaluated motor, cardiac or biochemical parameters. Conclusion: Our data suggest that intravenous administration of recombinant Phα1β may be feasible for drug-induced analgesia, without causing any severe side effects. |
URI : | http://www.repositorio.ufop.br/jspui/handle/123456789/15751 |
metadata.dc.identifier.doi: | https://doi.org/10.1590/1678-9199-JVATITD-2019-0070 |
ISSN : | 1678-9199 |
metadata.dc.rights.license: | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http:// creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Fonte: o PDF do artigo. |
Aparece en las colecciones: | DEFAR - Artigos publicados em periódicos |
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