Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/1417
Title: Follow-up of experimental chronic Chagas’ disease in dogs : use of polymerase chain reaction (PCR) compared with parasitological and serological methods.
Authors: Araújo, Flávio Marcos Gomes de
Bahia, Maria Terezinha
Magalhães, Neuza Maria de
Martins Filho, Olindo Assis
Veloso, Vanja Maria
Carneiro, Cláudia Martins
Tafuri, Washington Luiz
Lana, Marta de
Keywords: Chronic infection
Parasitological tests and serology
Trypanosoma cruzi
Dog
Issue Date: 2002
Citation: ARAÚJO, F. M. G. de et al. Follow-up of experimental chronic Chagas’ disease in dogs : use of polymerase chain reaction (PCR) compared with parasitological and serological methods. Acta Tropica, v. 81, n. 1, p. 21-31, jan. 2002. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0001706X01001966>. Acesso em: 25 set. 2012.
Abstract: In this study, the polymerase chain reaction (PCR) was compared with parasitological and serological methods to detect the infection in dogs, 5–12 years after experimental infection with Trypanosoma cruzi. The ability of parasitological methods to identify a positive animal was 22 and 11% by hemoculture and xenodiagnosis/xenoculture, respectively. On the other hand, the serological tests, including conventional serology and anti-live trypomastigote antibodies (ALTA) were positive in all infected dogs. Despite its low sensitivity, if considering only one reaction, the PCR analysis showed 100% of positivity, demonstrating the presence of parasite kDNA in all infected dogs. To identify a positive dog required at least two blood samples and up to nine repeated reactions using the same sample. Serial blood sample collection, ranging from 1 to 9, revealed that the percentage of dogs with positive PCR ranged from 67 to 100%. These findings suggested that, although the PCR is useful to detect the parasite in infected hosts, it should not be used isolated for the diagnosis of Chagas’ disease and warn for the necessity of serial blood collection and re-tests. Moreover, these data validate once more the dog as a model for Chagas’ disease since they demonstrate the permanence of infection by PCR, parasitological and serological methods, reaching relevant requisites for an ideal model to study this disease.
URI: http://www.repositorio.ufop.br/handle/123456789/1417
ISSN: 0001706X
metadata.dc.rights.license: O periódico Acta Tropica concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3282590943953.
Appears in Collections:DEACL - Artigos publicados em periódicos

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