Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/1410
Title: Further genetic characterization of the two Trypanosoma cruzi Berenice strains (Be-62 and Be-78) isolated from the first human case of Chagas disease (Chagas, 1909).
Authors: Cruz, Ruth Elizabeth
Macedo, Andréa Mara
Barnabé, Christian
Freitas, Jorge Marcelo de
Chiari, Egler
Veloso, Vanja Maria
Carneiro, Cláudia Martins
Bahia, Maria Terezinha
Tafuri, Washington Luiz
Lana, Marta de
Keywords: Isoenzyme
Microssatellites
Trypanosoma cruzi
Berenice strains
Issue Date: 2006
Citation: CRUZ, R. E. et al. Further genetic characterization of the two Trypanosoma cruzi Berenice strains (Be-62 and Be-78) isolated from the first human case of Chagas disease (Chagas, 1909). Acta Tropica, v. 97, n. 3, p. 239-46, mar. 2006. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0001706X05002500>. Acesso em: 20 set. 2012.
Abstract: We describe here an extension of a previous genetic characterization of Trypanosoma cruzi strains (Be-62 and Be-78) isolated from the patient Berenice, the first human case of Chagas disease [Chagas, C., 1909. Nova Tripanom´ıase humana. Estudos sobre morfologia e o ciclo evolutivo do Schizotrypanum cruzi, n. gen., n. sp., agente etiol´ojico da nova entidade morbida do homem. Mem. Inst. Oswaldo Cruz 1, 159–218]. We wanted to verify the composition of T. cruzi populations originated from these two isolates. In the present work, 22 enzymatic loci (MLEE), nine RAPD primers and 7 microsatellite loci were analyzed. Clones from both strains were also characterized to verify whether these strains are mono or polyclonal. Be-62 and Be-78 strains were different in 3 out of 22 enzymatic systems, in 3 out of 9 RAPD primers tested and in all microsatellite loci investigated. However, our data suggests that both strains are phylogenetically closely related, belonging to genetic group 32 from Tibayrenc and Ayala [Tibayrenc, M., Ayala, F.J., 1988. Isoenzime variability in Trypanosoma cruzi, the agent of Chagas’ disease: genetical, taxonomical, and epidemiological significance. Evolution 42, 277–292], equivalent to zymodeme 2 and T. cruzi II major lineage which, in Brazil, comprises parasites from the domestic cycle of the disease. Microsatellite analyses showed differences betweenthe parental strains but suggested that both populations are monoclonal since each strain and their respective clones showed the same amplification products.
URI: http://www.repositorio.ufop.br/handle/123456789/1410
ISSN: 0001706X
metadata.dc.rights.license: O periódico Acta Tropica concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3281990582397.
Appears in Collections:DEACL - Artigos publicados em periódicos

Files in This Item:
File Description SizeFormat 
ARTIGO_FurtherGeneticCharacterization.pdf205,62 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.