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Title: Extraction and fractionation effects on antiplasmodial activity and phytochemical composition of Palicourea hoffmannseggiana
Authors: Ohashi, Leticia Hiromi
Gontijo, Douglas da Costa
Nascimento, Maria Fernanda Alves do
Margalho, Luciano Ferreira
Brandão, Geraldo Célio
Oliveira, Alaíde Braga de
Keywords: Rubiaceae
Monoterpene indole alkaloids
β-carboline alkaloids
Plasmodium falciparum
Issue Date: 2021
Citation: OHASHI, L. H. et al. Extraction and fractionation effects on antiplasmodial activity and phytochemical composition of Palicourea hoffmannseggiana. Planta Medica International Open, v. 8, p. e34-e42, 2021. Disponível em: <>. Acesso em: 10 jun. 2021.
Abstract: The present study on Palicourea hoffmannseggiana, which was collected at Marapanim, state of Pará, Brazil, comprises the preparation of different stem and leaf extracts and fractions. Ethanol, hydroethanol, and water extracts were prepared by several methods and evaluated for in vitro activity against resistant Plasmodium falciparum (W2 strain), disclosing a low parasite growth inhibition effect ( < 50 %). Dereplication by UPLC-DAD-ESI −MS of the leaf ethanol extract showed the presence of two known alkaloids, lyalosidic and strictosidinic acids, along with a sinapoyl ester of lyalosidic acid, with m/z 719.33 [M +H] + , which is possibly a new monoterpene indole alkaloid representative. Sequential liquid-liquid acid-base alkaloid separations from the leaf ethanol extract as well as directly from leaf powder afforded fractions of increased parasite growth inhibition, reaching up to 92.5±0.7%. The most bioactive fractions were shown to contain the β-carboline alkaloids harmane and 4-methyl-β-carboline, along with N-methyl-tryptamine and N-acetyl-tryptamine, while monoterpene indole alkaloids were detected in inactive fractions of these processes. The present results demonstrate that these preliminary fractionation methods can lead to significantly active fractions supporting an adequate scale-up to carrying out the isolation of anti-plasmodial compounds.
ISSN: 2509-9264
metadata.dc.rights.license: This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons. org/licenses/by-nc-nd/4.0/). Fonte: o PDF do artigo.
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