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Title: Leishmania infantum amastin protein incorporated in distinct adjuvant systems induces protection against visceral leishmaniasis.
Authors: Ribeiro, Patrícia Aparecida Fernandes
Vale, Danniele Luciana
Dias, Daniel Silva
Lage, Daniela Pagliara
Mendonça, Débora Vasconcelos Costa
Ramos, Fernanda Fonseca
Carvalho, Lívia Mendes
Carvalho, Ana Maria Ravena Severino
Steiner, Bethina Trevisol
Roque, Marjorie Coimbra
Silva, João Augusto Oliveira da
Oliveira, Jamil Silvano de
Tavares, Grasiele de Sousa Vieira
Galvani, Nathália Coral
Martins, Vivian Tamietti
Chávez Fumagalli, Miguel Angel
Roatt, Bruno Mendes
Moreira, Ricardo Luiz Fontes
Souza, Daniel Menezes
Oliveira, Mônica Cristina de
Ávila, Ricardo Andrez Machado de
Teixeira, Antonio Lucio
Coelho, Eduardo Antônio Ferraz
Keywords: Vaccine
Issue Date: 2020
Citation: RIBEIRO, P. A. F. et al. Leishmania infantum amastin protein incorporated in distinct adjuvant systems induces protection against visceral leishmaniasis. Cytokine, v. 129, artigo 155031, maio 2020. Disponível em: <>. Acesso em: 10 jun. 2021.
Abstract: The control measures against visceral leishmaniasis (VL) include a precise diagnosis of disease, the treatment of human cases, and reservoir and vector controls. However, these are insufficient to avoid the spread of the disease in specific countries worldwide. As a consequence, prophylactic vaccination could be interesting, although no effective candidate against human disease is available. In the present study, the Leishmania infantum amastin protein was evaluated regarding its immunogenicity and protective efficacy against experimental VL. BALB/c mice immunized with subcutaneous injections of the recombinant protein with or without liposome/saponin (Lip/Sap) as an adjuvant. After immunization, half of the animals per group were euthanized and immunological evaluations were performed, while the others were challenged with L. infantum promastigotes. Forty-five days after infection, the animals were euthanized and parasitological and immunological evaluations were performed. Results showed the development of a Th1-type immune response in rAmastin-Lip and rAmastin-Sap/vaccinated mice, before and after infection, which was based on the production of protein and parasite-specific IFN-γ, IL-12, GM-CSF, and nitrite, as well as the IgG2a isotype antibody. CD4+ T cells were mainly responsible for IFN-γ production in vaccinated mice, which also presented significant reductions in parasitism in their liver, spleen, draining lymph nodes, and bone marrow. In addition, PBMC cultures of treated VL patients and healthy subjects stimulated with rAmastin showed lymphoproliferation and higher IFN-γ production. In conclusion, the present study shows the first case of an L. infantum amastin protein associated with distinct delivery systems inducing protection against L. infantum infection and demonstrates an immunogenic effect of this protein in human cells.
ISSN: 1043-4666
Appears in Collections:DECBI - Artigos publicados em periódicos

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