Please use this identifier to cite or link to this item:
Title: Antiviral effect of silymarin against Zika virus in vitro.
Authors: Silva, Tales Fernando da
Ferraz, Ariane Coelho
Almeida, Letícia Trindade
Caetano, Camila Carla da Silva
Camini, Fernanda Caetano
Lima, Rafaela Lameira Souza
Andrade, Ana Cláudia dos Santos Pereira
Oliveira, Danilo Bretas de
Rocha, Kamila Lorene Soares
Silva, Breno de Mello
Magalhães, José Carlos de
Magalhães, Cíntia Lopes de Brito
Issue Date: 2020
Citation: SILVA, T. F. da et al. Antiviral effect of silymarin against Zika virus in vitro. Acta Tropica, v. 211, artigo 105613, nov. 2020. Disponível em: <>. Acesso em: 10 jun. 2021.
Abstract: Zika virus (ZIKV) epidemic and its association with severe neurological syndromes have raised worldwide concern. Despite the great clinical relevance of this infection, no vaccine or specific treatment is available and the search for antiviral compounds against ZIKV is extremely necessary. Several natural compounds, such as silymarin, exhibit antioxidant, hepatoprotective, and antiviral properties; however, the antiviral potential of this compound remains partially investigated. Therefore, the objective of this study was to evaluate in vitro the antiviral activity of silymarin against ZIKV infection. Global antiviral activity, dose-dependent, plaque reduction, and time-of-drug-addition assays were used to determine the anti-ZIKV activity of silymarin. Additionally, to start characterizing the mechanisms of action we determined whether silymarin could have a virucidal effect and inhibit viral adsorption and penetration stages. Regarding its global antiviral activity, silymarin showed significant inhibition of ZIKV infection, protecting cells infected with EC50 equal to 34.17μg/mL, with a selectivity index greater than 17 and 4x greater than that of the positive control (ribavirin). Its greatest efficiency was achieved at 125μg/mL, whose cell viability did not differ from the control without infection and treatment. Furthermore, treatment with silymarin reduced viral load by up to two logs (> 90%) concerning viral control, when evaluating virucidal activity and the precocious times of infection. Thus, our results set to show the promising anti-ZIKV activity of silymarin, which does not seem to have a single inhibition mechanism, acting at different times of infection, and still has the advantage of silymarin be a phytotherapy already available on the market.
ISSN: 0001-706X
Appears in Collections:DECBI - Artigos publicados em periódicos

Files in This Item:
File Description SizeFormat 
  Restricted Access
645,02 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.