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Title: Zika virus induces oxidative stress and decreases antioxidant enzyme activities in vitro and in vivo.
Authors: Almeida, Letícia Trindade
Ferraz, Ariane Coelho
Caetano, Camila Carla da Silva
Menegatto, Marília Bueno da Silva
Andrade, Ana Cláudia dos Santos Pereira
Lima, Rafaela Lameira Souza
Camini, Fernanda Caetano
Pereira, Samille Henriques
Pereira, Karla Yanca da Silva
Silva, Breno de Mello
Perucci, Luiza Oliveira
Silva, André Talvani Pedrosa da
Magalhães, José Carlos de
Magalhães, Cíntia Lopes de Brito
Issue Date: 2020
Citation: ALMEIDA, L. T. et al. Zika virus induces oxidative stress and decreases antioxidant enzyme activities in vitro and in vivo. Virus Research, v. 286, id 198084, 2020. Disponível em: <>. Acesso em: 10 jun. 2021.
Abstract: The first outbreak of Zika virus (ZIKV) infection in the Americas, especially in Brazil, was reported in 2015. Fever, headache, rash, and conjunctivitis are the common symptoms of ZIKV infection. Unexpected clinical outcomes, such as microcephaly and Guillain-Barré syndrome, have also been reported. The recent spread of ZIKV and its association with severe illness has created an urgent need to understand its pathogenesis and find potential therapeutic targets. Studies show that some viruses, including Flavivirus, trigger oxidative stress, which affects cellular metabolism, viral cycle, and pathogenesis. However, the role of oxidative stress in ZIKV infection needs to be investigated. Here, we analyzed ZIKV infection-triggered oxidative stress and modified antioxidant enzyme activities. U87-MG and HepG2 cells were infected to measure reactive oxygen species (ROS), malondialdehyde (MDA), and carbonyl protein levels, the activities of superoxide dismutase (SOD) and catalase (CAT), and the activation of nuclear factor erythroid 2p45-related factor 2 (Nrf2). ZIKV infection induced a significant increase in ROS, lipid peroxidation, and protein carbonylation products and a significant decrease in SOD and CAT activities accompanied by inhibition of Nrf2 activation in both cell lines. Further, MDA and carbonyl protein levels and SOD and CAT activities were evaluated in the brain and liver of ZIKV-infected C57BL/6 mice, and oxidative stress associated with antioxidant depletion was also found to occur in vivo. Together, our findings indicate the potential use of antioxidants as a novel therapeutic approach to Zika disease, and future studies in this direction are warranted.
ISSN: 0168-1702
Appears in Collections:DECBI - Artigos publicados em periódicos

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