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Title: Reuse of hot trub as an active ingredient with antioxidant and antimicrobial potential.
Authors: Costa, Fernanda Senna Ferreira
Amparo, Tatiane Roquete
Seibert, Janaína Brandão
Silveira, Benila Maria
Silva, Regislainy Gomes da
Pereira, Deise Inocêncio
Barbosa, Ramon Gontijo Garcia
Santos, Orlando David Henrique dos
Brandão, Geraldo Célio
Teixeira, Luiz Fernando de Medeiros
Vieira, Paula Melo de Abreu
Souza, Gustavo Henrique Bianco de
Issue Date: 2020
Citation: COSTA, F. S. F. et al. Reuse of hot trub as an active ingredient with antioxidant and antimicrobial potential. Waste and Biomass Valorization, v. 12, p. 2037-2047, 2020. Disponível em: <>. Acesso em: 10 jun. 2021.
Abstract: The reuse of agro-industrial residues is vital to minimize their environmental impact and alternatives for their application are needed. In this research, we evaluated the chemical composition as well as antioxidant and antibacterial activities of the extract from hot trub, that is the second solid waste obtained during the brewing process. Total phenolic and favonoids compounds were determined by colorimetric assays and the compounds were identifed by UHPLC-DAD-MS/MS. Antioxidant activity was evaluated by ABTS and DPPH methods. Antibacterial activity against Gram-positive and Gram-negative bacteria was analyzed by agar difusion and microdilution methods. Cytotoxicity was evaluated against murine fbroblast L929 cell line by MTT assay. The results showed that the content of phenolic and favonoid compounds ranged according to the fractions from 8.237 to 13.390 mg GAE/g and from 0.019 to 1.643 mg QE/g, respectively. The compounds cohumulone, cohulupone, hulupone/adhulupone, isoxanthohumol, xanthohumol, hydroxytricyclocolupone epimers, hydroxytricyclolupone/ hydroxytricicloadlupone epimers and nortricyclocolupone were suggested. Higher antioxidant activity was observed for the crude ethanol extract. The hexane fraction presented the lowest MIC value against Gram-positive bacteria, as well as lower cytotoxicity. These results are promising and indicate a great potential for the reuse of this byproduct as a source of natural antioxidant and for the microbiological applications, which consequently would reduce the environmental impact.
ISSN: 1877-265X
Appears in Collections:DEACL - Artigos publicados em periódicos

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