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Title: In vitro infectivity of strains isolated from dogs naturally infected with Leishmania infantum present a distinct pathogenic profile in hamsters.
Authors: Resende, Lucilene Aparecida
Soares, Rodrigo Dian de Oliveira Aguiar
Moreira, Nádia das Dores
Ferreira, Sidney de Almeida
Lanna, Mariana Ferreira
Cardoso, Jamille Mirelle de Oliveira
Mathias, Fernando Augusto Siqueira
Vital, Wendel Coura
Mariano, Reysla Maria da Silveira
Leite, Jaqueline Costa
Silveira, Patricia
Carvalho, Tatiane Furtado de
Santos, Renato Lima
Lemos, Denise da Silveira
Martins Filho, Olindo Assis
Dutra, Walderez Ornelas
Reis, Alexandre Barbosa
Giunchetti, Rodolfo Cordeiro
Keywords: Visceral leishmaniasis
Experimental infection
Issue Date: 2020
Citation: RESENDE, L. A. et al. In vitro infectivity of strains isolated from dogs naturally infected with Leishmania infantum present a distinct pathogenic profile in hamsters. Frontiers in Medicine, v. 7, artigo 496, ago. 2020. Disponível em: <>. Acesso em: 10 jun. 2021.
Abstract: Visceral leishmaniasis (VL) is a severe disease caused by Leishmania infantum. Dogs are the parasite’s main reservoir, favoring its transmission in the urban environment. The analysis of L. infantum from infected dogs contributes to the identification of more virulent parasites, thereby supporting basic and applied studies such as vaccinal and therapeutic strategies. We proposed the in vitro and in vivo characterization of L. infantum strains from naturally infected dogs from a VL endemic area based on an infectivity and pathogenicity analysis. DH82 canine macrophages were infected in vitro with different strains for infectivity analysis, showing distinct infectivity profiles. The strains that showed greater and lesser infectivity using in vitro analyses (616 and 614, respectively) were used to infect hamsters for pathogenicity analysis. The group infected with strain 616 showed 100% survival while the group infected with strain 614 showed 50% after seven months of follow up. Furthermore, the 614 strain induced more noticeable clinicopathological changes and biochemical abnormalities in liver function, along with high inflammation and parasite load in the liver and spleen. We confirmed high variability of infectivity and pathogenicity in L. infantum strains from infected dogs. The results support the belief that screening for L. infantum infectivity using in vitro experiments is inadequate when it comes to selecting the most pathogenic strain.
ISSN: 2296-858X
metadata.dc.rights.license: This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Fonte: o PDF do artigo.
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