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Title: Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis.
Authors: Cordeiro, Taís Aparecida Reis
Martins, Helen Rodrigues
Franco, Diego Leoni
Santos, Fred Luciano Neves
Celedon, Paola Alejandra Fiorani
Cantuária, Vinícius Lopes
Lana, Marta de
Reis, Alexandre Barbosa
Ferreira, Lucas Franco
Keywords: Dual detection
Issue Date: 2020
Citation: CORDEIRO, T. A. R. et al. Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis. Biosensors & Bioelectronics, v. 169, p. 112573, 2020. Disponível em: <>. Acesso em: 10 jun. 2021.
Abstract: In this work, a dual detection system based on an impedimetric immunosensor was developed for the first time for the simultaneous detection of anti-Trypanosoma cruzi and anti-Leishmania infantum antibodies in human and dog serum samples. The IBMP 8.1 and rLci1A/rLci2B recombinant antigens were immobilized over the surface of dual screen-printed carbon electrodes (W1 and W2) modified with poly (4-hydroxyphenylacetic acid). Under optimized conditions, the immunosensor recognized specific interactions for anti-T. cruzi antibodies up to a dilution of 1:10,240 and for anti-L. infantum up to 1:5120 in canine serum samples. Relative standard deviation (RSD) values of 2.8% for W1 and 3.6% for W2 were obtained for T. cruzi (W1) and L. infantum antigen (W2) samples in three different electrodes for 3 days (n = 9). The immunosensor was stored at 4 ◦C for 8 weeks, with activity retention of 70.2% in W1 and 78.2% in W2. The results using the recombinant proteins revealed that all antigens discriminated between negative and positive samples (p < 0.0001) in both dog and human groups, as well as no cross-reactivity could be detected among sera with other infections. With this approach, immunosensor-based diagnostic tests achieved 100% accuracy, suggesting that the antigens are eligible to enter Phase-II studies.
ISSN: 0956-5663
Appears in Collections:DEACL - Artigos publicados em periódicos

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