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Title: The golden mussel proteome and its response to niclosamide : uncovering rational targets for control or elimination.
Authors: Sanson, Ananda Lima
Cosenza Contreras, Miguel de Jesus
Marco, Ricardo De
Neves, Leandro Xavier
Mattei, Bruno
Silva, Gustavo Gonçalves
Magalhães, Paulo Henrique Vieira
Andrade, Milton Hércules Guerra de
Borges, William de Castro
Keywords: Limnoperna fortunei
Proteomic analysis
Issue Date: 2020
Citation: SANSON, A. L. et al. The golden mussel proteome and its response to niclosamide: uncovering rational targets for control or elimination. Journal of Proteomics, v. 217, abr. 2020. Disponível em: <>. Acesso em: 10 mar. 2020.
Abstract: The Asian invasive species Limnoperna fortunei (Dunker, 1857), known as the golden mussel, causes great economic and environmental damage due to its fixative capacity and accelerated proliferation. Molecular studies for the control of larval and adult forms are of great economic, scientific and technological interest. Here, we first report on the compositional analysis of the L. fortunei proteome obtained through shotgun analysis using LC-MS/ MS. Among those 2790 proteins identified, many of them related to secretory processes and membrane receptors. Our second approach consisted in exposing the mollusc to the molluscicide niclosamide to evaluate the induced proteomic alterations. Exposure to niclosamide at 0.25 mg/L for 24 h resulted in a pronounced differential abundance of proteins when compared to those obtained when exposure was reduced to 4 h at 2.3 mg/ L. In total, 342 proteins were found differentially expressed in the responsive individuals as revealed by labelfree quantitative proteomics. Regarding the affected cell processes were: cell division and differentiation, cytoskeletal organization and compartment acidification (upregulated), and energy metabolism (downregulated). Our findings constitute the first inventory of the expressed proteome of the golden mussel and have the potential to contribute with a more rational proposition of molecular targets for control and monitoring of this species. Significance: With the recent availability of transcriptomic and genomic data applied to L. fortunei the timing is right to interrogate its putative gene repertoire using proteomic techniques. These have the potential to validate the existence of the predicted genes, infer their relative abundance and quantify their levels as a response to environmental stressors or various agents. Here we provided an inventory of the golden mussel proteome and evaluated its response to the molluscicide niclosamide. The obtained results open new avenues for intervention aimed at its control or elimination, particularly by targeting the various cellular processes that were uncovered.
ISSN: 1874-3919
Appears in Collections:DEMEC - Artigos publicados em periódicos

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