Use este identificador para citar ou linkar para este item:
http://www.repositorio.ufop.br/jspui/handle/123456789/12221
Título: | Cytogenetic analysis and chromosomal mapping of repetitive DNA in Melipona species (Hymenoptera, Meliponini). |
Autor(es): | Travenzoli, Natália Martins Lima, Bárbara Soares Amoroso Cardoso, Danon Clemes Santos, Jorge Abdala Dergam dos Salomão, Tânia Maria Fernandes Lopes, Denilce Meneses |
Palavras-chave: | Fluorescence in situ hybridization Euchromatin Heterochromatin Molecular cytogenetics Nucleotide constitution |
Data do documento: | 2019 |
Referência: | TRAVENZOLI, N. M. et al. Cytogenetic analysis and chromosomal mapping of repetitive DNA in Melipona species (Hymenoptera, Meliponini). Cytogenetic and Genome Research, v. 158, n. 4, p. 213–224, set. 2019. Disponível em: <https://www.karger.com/Article/FullText/501754>. Acesso em: 10 mar. 2020. |
Resumo: | Stingless bees of the genus Melipona are subdivided into 4 subgenera called Eomelipona, Melikerria, Melipona sensu stricto, and Michmelia according to species morphology. Cytogenetically, the species of the genus Melipona show variation in the amount and distribution of heterochromatin along their chromosomes and can be separated into 2 groups: the first with low content of heterochromatin and the second with high content of heterochromatin. These heterochromatin patterns and the number of chromosomes are characteristics exclusive to Melipona karyotypes that distinguish them from the other genera of the Meliponini. To better understand the karyotype organization in Melipona and the relationship among the subgenera, we mapped repetitive sequences and analyzed previously reported cytogenetic data with the aim to identify cytogenetic markers to be used for investigating the phylogenetic relationships and chromosome evolution in the genus. In general, Melipona species have 2n = 18 chromosomes, and the species of each subgenus share the same characteristics in relation to heterochromatin regions, DAPI/CMA3 fluorophores, and the number and distribution of 18S rDNA sites. Microsatellites were observed only in euchromatin regions, whereas the (TTAGG)6 repeats were found at telomeric sites in both groups. Our data indicate that in addition to the chromosome number, the karyotypes in Melipona could be separated into 2 groups that are characterized by conserved cytogenetic features and patterns that generally are shared by species within each subgenus, which may reflect evolutionary constraints. Our results agree with the morphological separation of the Melipona into 4 subgenera, suggesting that they must be independent evolutionary lineages. |
URI: | http://www.repositorio.ufop.br/handle/123456789/12221 |
Link para o artigo: | https://www.karger.com/Article/FullText/501754 |
DOI: | https://doi.org/10.1159/000501754 |
ISSN: | 1424-859X |
Aparece nas coleções: | DEBIO - Artigos publicados em periódicos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
---|---|---|---|---|
ARTIGO_CytogeneticAnalysisChromosomal.pdf Restricted Access | 3,81 MB | Adobe PDF | Visualizar/Abrir |
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.