Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/12178
Title: Effect on cellular recruitment and the innate immune response by combining saponin, monophosphoryl lipid-A and incomplete freund’s adjuvant with Leishmania (Viannia) braziliensis antigens for a vaccine formulation.
Authors: Souza, Juliana Vitoriano de
Mathias, Fernando Augusto Siqueira
Moreira, Nádia das Dores
Soares, Rodrigo Dian de Oliveira Aguiar
Vieira, Paula Melo de Abreu
Carvalho, Andréa Teixeira de
Carneiro, Cláudia Martins
Giunchetti, Rodolfo Cordeiro
Brito, Rory Cristiane Fortes de
Fujiwara, Ricardo Toshio
Roatt, Bruno Mendes
Melo, Maria Norma
Reis, Alexandre Barbosa
Keywords: Cellular infiltration
Cytokines
Issue Date: 2019
Citation: SOUZA, J. V. et al. Effect on cellular recruitment and the innate immune response by combining saponin, monophosphoryl lipid-A and incomplete freund’s adjuvant with Leishmania (Viannia) braziliensis antigens for a vaccine formulation. Vaccine, v. 37, p. 7269-7279, nov. 2019. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0264410X19312952?via%3Dihub>. Acesso em: 10 fev. 2020.
Abstract: The poor immunogenicity displayed by some antigens has encouraged the development of strategies to improve the immune response and safety of vaccine candidates, resulting in an intense search for substances that potentiate vaccine response. Adjuvants have these properties helping vaccine candidates to induce a strong, durable, and fast immune response. In this study, we evaluated the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund’s Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE ) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL. The specific immune response induced by these compositions demonstrated that they were powerfully immunogenic, increasing cellular infiltration in the skin. Draining lymph nodes cultures showed that LBIFA and LBMPL have higher ability to increase the capacity of APCs to present antigens, with increased frequency of CD11c+ CD86+ cells. SAP, MPL, LBSAP, LBIFA and LBMPL could activate lymphocytes increasing expression of CD69 and CD25. LBSAP group was an excellent inducer of pro-inflammatory cytokines at 24 h. At 48 h, higher cytokines production was observed in IFA, LBIFA, MPL and LBMPL groups. Our data demonstrate that LBSAP and LBMPL are potential formulations to be tested in other experimental models. Also, the data obtained could expand the knowledge about immune response after sensitization and also contribute to the development of safe, immunogenic and effective vaccines.
URI: http://www.repositorio.ufop.br/handle/123456789/12178
metadata.dc.identifier.uri2: https://www.sciencedirect.com/science/article/pii/S0264410X19312952?via%3Dihub
metadata.dc.identifier.doi: https://doi.org/10.1016/j.vaccine.2019.09.067
ISSN: 2076-393X
Appears in Collections:DEACL - Artigos publicados em periódicos

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