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Título : Validation of a colorimetric assay for the in vitro screening of inhibitors of angiotensin-converting enzyme (ACE) from plant extracts.
Autor : Serra, Carla Penido
Côrtes, Steyner de França
Lombardi, Júlio Antônio
Oliveira, Alaíde Braga de
Braga, Fernão Castro
Palabras clave : In vitro colorimetric assay
Plant extracts
Screening
Angiotensin-converting enzyme inhibitors
Fecha de publicación : 2005
Citación : SERRA, C. P. et al. Validation of a colorimetric assay for the in vitro screening of inhibitors of angiotensin-converting enzyme (ACE) from plant extracts. Phytomedicine, v. 12, n. 6-7, p. 424-432, jun. 2005. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0944711305000590>. Acesso em: 30 jul. 2012.
Resumen : Anew method for the in vitro screening of plant extracts with potential angiotensin-converting enzyme (ACE) inhibitory activity is proposed. The method is based on the cleavage of the substrate hippuryl-glycyl-glycine by ACE and subsequent reaction with trinitrobenzenesulfonic acid to form 2,4,6-trinitrophenyl-glycyl-glycine, whose absorbance is determined at 415nm in a microtitre plate reader. Rabbit lung dehydrated by acetone was employed as an enzyme source. Validation of the method showed satisfactory intra-day (CV ¼ 7.63%) and inter-day precision (CV ¼ 13.61%), recovery (97–102.1%), sensitivity (IC50 ¼ 14.1 nmol/l) and linearity in the range 7.5–120 mmol/l of glycyl-glycine (r2 ¼ 0:9921). Besides, the method showed good correlation with a HPLC assay already established for the screening of ACE inhibitors (r ¼ 0:9935 and 0:9034; respectively, for captopril solutions and for plant extracts). The method involves only inexpensive reagents and apparatus.
URI : http://www.repositorio.ufop.br/handle/123456789/1215
ISSN : 09447113
metadata.dc.rights.license: O periódico Phytomedicine concede permissão ao repositório Instucional da UFOP para depositar este artigo. Número da licença: 3233050124767.
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