Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/jspui/handle/123456789/12020
Title: XPD/ERCC2 mutations interfere in cellular responses to oxidative stress.
Authors: Lerner, Leticia Koch
Moreno, Natália Cestari
Rocha, Clarissa Ribeiro Reily
Munford, Veridiana
Santos, Valquíria Tiago dos
Soltys, Daniela Tathiana
Garcia, Camila Carrião Machado
Sarasin, Alain
Menck, Carlos Frederico Martins
Issue Date: 2019
Citation: LERNER, L. K. et al. XPD/ERCC2 mutations interfere in cellular responses to oxidative stress. Mutagenesis, v. 34, n. 4, p 341–354, jul. 2019. Disponível em: <https://academic.oup.com/mutage/article/34/4/341/5539652>. Acesso em: 10 fev. 2020.
Abstract: Nucleotide excision repair (NER) is a conserved, flexible mechanism responsible for the removal of bulky, helix-distorting DNA lesions, like ultraviolet damage or cisplatin adducts, but its role in the repair of lesions generated by oxidative stress is still not clear. The helicase XPD/ERCC2, one of the two helicases of the transcription complex IIH, together with XPB, participates both in NER and in RNA pol II-driven transcription. In this work, we investigated the responses of distinct XPDmutated cell lines to the oxidative stress generated by photoactivated methylene blue (MB) and KBrO3 treatments. The studied cells are derived from patients with XPD mutations but expressing different clinical phenotypes, including xeroderma pigmentosum (XP), XP and Cockayne syndrome (XP-D/CS) and trichothiodystrophy (TTD). We show by different approaches that all XPD-mutated cell lines tested were sensitive to oxidative stress, with those from TTD patients being the most sensitive. Host cell reactivation (HCR) assays showed that XP-D/CS and TTD cells have severely impaired repair capacity of oxidised lesions in plasmid DNA, and alkaline comet assays demonstrated the induction of significantly higher amounts of DNA strand breaks after treatment with photoactivated MB in these cells compared to wild-type cells. All XPD-mutated cells presented strong S/G2 arrest and persistent γ-H2AX staining after photoactivated MB treatment. Taken together, these results indicate that XPD participates in the repair of lesions induced by the redox process, and that XPD mutations lead to differences in the response to oxidatively induced damage.
URI: http://www.repositorio.ufop.br/handle/123456789/12020
metadata.dc.identifier.uri2: https://academic.oup.com/mutage/article/34/4/341/5539652
metadata.dc.identifier.doi: https://doi.org/10.1093/mutage/gez020
ISSN: 1464-3804
Appears in Collections:DECBI - Artigos publicados em periódicos

Files in This Item:
File Description SizeFormat 
ARTIGO_XPDERCC2Mutations.pdf
  Restricted Access
1,11 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.