Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/jspui/handle/123456789/12002
Title: Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) delays egg hatching and larval development of progeny.
Authors: Chaves, Bárbara Aparecida
Vieira Junior, Ademir Bentes
Silveira, Karine Renata Dias
Paz, Andréia da Costa
Vaz, Evelyn Beatriz da Costa
Araújo, Raphaela Guedes Pereira
Rodrigues, Nilton Barnabé
Campolina, Thaís Bonifácio
Orfanó, Alessandra da Silva
Pimenta, Rafael Nacif
Villegas, Luis Eduardo Martínez
Melo, Fabrício Freire de
Silva, Breno de Mello
Monteiro, Wuelton Marcelo
Guerra, Maria das Graças Vale Barbosa
Lacerda, Marcus Vinícius Guimarães de
Norris, Douglas Eric
Secundino, Nagila Francinete Costa
Pimenta, Paulo Filemon Paolucci
Keywords: Fitness cost
Issue Date: 2019
Citation: CHAVES, B. A. et al. Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) delays egg hatching and larval development of progeny. Journal of Medical Entomology, v. 56, n. 6, p. 1739–1744, nov. 2019. Disponível em: <https://academic.oup.com/jme/article/56/6/1739/5529105>. Acesso em: 10 fev. 2020.
Abstract: Zika virus (ZIKV) has emerged as a globally important arbovirus and has been reported from all states of Brazil. The virus is primarily transmitted to humans through the bite of an infective Aedes aegypti (Linnaeus, 1762) or Aedes albopictus (Skuse, 1895). However, it is important to know if ZIKV transmission also occurs from Ae. aegypti through infected eggs to her offspring. Therefore, a ZIKV and dengue virus (DENV) free colony was established from eggs collected in Manaus and maintained until the third–fourth generation in order to conduct ZIKV vertical transmission (VT) experiments which used an infectious bloodmeal as the route of virus exposure. The eggs from ZIKV-infected females were allowed to hatch. The resulting F1 progeny (larvae, pupae, and adults) were quantitative polymerase chain reaction (qPCR) assayed for ZIKV. The viability of ZIKV vertically transmitted to F1 progeny was evaluated by cultivation in C6/36 cells. The effects of ZIKV on immature development of Ae. aegypti was assessed and compared with noninfected mosquitoes. Amazonian Ae. Aegypti were highly susceptible to ZIKV infection (96.7%), and viable virus passed to their progeny via VT. Moreover, eggs from the ZIKV-infected mosquitoes had a significantly lower hatch rate and the slowest hatching. In addition, the larval development period was slower when compared to noninfected, control mosquitoes. This is the first study to illustrate VT initiated by oral infection of the parental population by using mosquitoes, which originated from the field and a ZIKV strain that is naturally circulating in-country. Additionally, this study suggests that ZIKV present in the Ae. aegypti can modify the mosquito life cycle. The data reported here suggest that VT of ZIKV to progeny from naturally infected females may have a critical epidemiological role in the dissemination and maintenance of the virus circulating in the vector.
URI: http://www.repositorio.ufop.br/handle/123456789/12002
metadata.dc.identifier.doi: https://doi.org/10.1093/jme/tjz110
ISSN: 1938-2928
metadata.dc.rights.license: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/ by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. Fonte: o próprio artigo.
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