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Título : | The PLC1 encoded phospholipase C in the yeast Saccharomyces cerevisiae is essential for glucose-induced phosphatidylinositol turnover and activation of plasma membrane H -ATPase. |
Autor : | Coccetti, Paola Tisi, Renata Martegani, Enzo Teixeira, Leonardo Souza Brandão, Rogélio Lopes Castro, Ieso de Miranda Thevelein, Johan Maria |
Palabras clave : | Compound 48/80 Saccharomyces cerevisiae Glucose induction Signal transduction Medium acidification |
Fecha de publicación : | 1998 |
Citación : | COCCETTI, P. et al. The PLC1 encoded phospholipase C in the yeast Saccharomyces cerevisiae is essential for glucose-induced phosphatidylinositol turnover and activation of plasma membrane H -ATPase. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, v. 1405, n. 1-3, p. 147-154, out. 1998. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0167488998000998>. Acesso em: 19 jul. 2012. |
Resumen : | Addition of glucose to glucose-deprived cells of the yeast Saccharomyces cerevisiae triggers rapid turnover of phosphatidylinositol, phosphatidylinositol-phosphate and phosphatidylinositol 4,5-bisphosphate. Glucose stimulation of PI turnover was measured both as an increase in the specific ratio of 32P-labeling and as an increase in the level of diacylglycerol after addition of glucose. Glucose also causes rapid activation of plasma membrane H.-ATPase. We show that in a mutant lacking the PLC1 encoded phospholipase C, both processes were strongly reduced. Compound 48/80, a known inhibitor of mammalian phospholipase C, inhibits both processes. However, activation of the plasma membrane H.- ATPase is only inhibited by concentrations of compound 48/80 that strongly inhibit phospholipid turnover. Growth was inhibited by even lower concentrations. Our data suggest that in yeast cells, glucose triggers through activation of the PLC1 gene product a signaling pathway initiated by phosphatidylinositol turnover and involved in activation of the plasma membrane H.-ATPase. |
URI : | http://www.repositorio.ufop.br/handle/123456789/1179 |
ISSN : | 01674889 |
metadata.dc.rights.license: | O periódico Biochimica et Biophysica Acta. Molecular Cell Research concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3266591238516. |
Aparece en las colecciones: | DEFAR - Artigos publicados em periódicos |
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Fichero | Descripción | Tamaño | Formato | |
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ARTIGO_EncodedPhospholipaseYeast.pdf | 311,93 kB | Adobe PDF | Visualizar/Abrir |
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