Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/1164
Title: Carbonyl cyanidem - chlorophenylhydrazone induced calcium signaling and activation of plasma membrane H1-ATPase in the yeast Saccharomyces cerevisiae.
Authors: Pereira, Michele B. P.
Tisi, Renata
Fietto, Luciano Gomes
Cardoso, Anamaria de Souza
França, Mônica M.
Carvalho, Fernanda Machado de
Trópia, Maria José Magalhães
Martegani, Enzo
Castro, Ieso de Miranda
Brandão, Rogélio Lopes
Keywords: Depolarizing compounds
Saccharomyces cerevisiae
Issue Date: 2008
Citation: PEREIRA, M. B. P. et al. Carbonyl cyanidem - chlorophenylhydrazone induced calcium signaling and activation of plasma membrane H1-ATPase in the yeast Saccharomyces cerevisiae. FEMS Yeast Research, v. 8, n.4, p.622-630, jun. 2008. Disponível em: <http://onlinelibrary.wiley.com/doi/10.1111/j.1567-1364.2008.00380.x/pdf>. Acesso em: 18 jul. 2012.
Abstract: The plasma membrane H1-ATPase from Saccharomyces cerevisiae is an enzyme that plays a very important role in the yeast physiology. The addition of protonophores, such as 2,4-dinitrophenol (DNP) and carbonyl cyanide m-chlorophenylhydrazone (CCCP), also triggers a clear in vivo activation of this enzyme. Here, we demonstrate that CCCP-induced activation of the plasma membrane H1-ATPase shares some similarities with the sugar-induced activation of the enzyme. Phospholipase C and protein kinase C activities are essential for this activation process while Gpa2p, a G protein involved in the glucose-induced activation of the ATPase, is not required. CCCP also induces a phospholipase Cdependent increase in intracellular calcium. Moreover, we show that the availability of extracellular calcium is required for CCCP stimulation of H1-ATPase, suggesting a possible connection between calcium signaling and activation of ATPase.
URI: http://www.repositorio.ufop.br/handle/123456789/1164
ISSN: 15671364
metadata.dc.rights.license: O Periódico FEMS Yeast Research concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 500810576.
Appears in Collections:DEFAR - Artigos publicados em periódicos

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