Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/1161
Title: Protective effect of ions against cell death induced by acid stress in Saccharomyces.
Authors: Sant'Ana, Gilzeane dos Santos
Paes, Lisvane da Silva
Paiva, Argentino F. Vieira
Fietto, Luciano Gomes
Tótola, Antônio Helvécio
Trópia, Maria José Magalhães
Lemos, Denise da Silveira
Lucas, Cândida Manuel Ribeiro Simões
Fietto, Juliana Lopes Rangel
Brandão, Rogélio Lopes
Castro, Ieso de Miranda
Keywords: Acid tolerance
Probiotic
Issue Date: 2009
Citation: SANT'ANA, G. dos S. et al. Protective effect of ions against cell death induced by acid stress in Saccharomyces. FEMS Yeast Research, v. 9, n.5, p.701-712, ago. 2009. Disponível em: <http://onlinelibrary.wiley.com/doi/10.1111/j.1567-1364.2009.00523.x/pdf>. Acesso em: 18 jul. 2012.
Abstract: Saccharomyces boulardii is a probiotic used to prevent or treat antibiotic-induced gastrointestinal disorders and acute enteritis. For probiotics to be effective they must first be able to survive the harsh gastrointestinal environment. In this work, we show that S. boulardii displayed the greatest tolerance to simulated gastric environments compared with several Saccharomyces cerevisiae strains tested. Under these conditions, a pH 2.0 was the main factor responsible for decreased cell viability. Importantly, the addition of low concentrations of sodium chloride (NaCl) protected cells in acidic conditions more effectively than other salts. In the absence of S. boulardii mutants, the protective effects of Na1 in yeast viability in acidic conditions was tested using S. cerevisiae Na1-ATPases (ena1-4), Na1/H1 antiporter (nha1D) and Na1/H1 antiporter prevacuolar (nhx1D) null mutants, respectively. Moreover, we provide evidence suggesting that this protection is determined by the plasma membrane potential, once altered by low pH and low NaCl concentrations. Additionally, the absence or low expression/activity of Ena proteins seems to be closely related to the basal membrane potential of the cells.
URI: http://www.repositorio.ufop.br/handle/123456789/1161
ISSN: 15671364
metadata.dc.rights.license: O Periódico FEMS Yeast Research concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 500810556
Appears in Collections:DEFAR - Artigos publicados em periódicos

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