Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/11099
Title: Evaluation of three recombinant proteins for the development of ELISA and immunochromatographic tests for visceral leishmaniasis serodiagnosis.
Authors: Santos, Anna Raquel Ribeiro dos
Serufo, Ângela Vieira
Figueiredo, Maria Marta
Godoi, Lara Carvalho
Vitório, Jéssica Gardone
Marcelino, Andreza Pain
Avelar, Daniel Moreira de
Rodrigues, Fernandes Tenório Gomes
Coelho, George Luiz Lins Machado
Medeiros, Fernanda Alvarenga Cardoso
Jeronimo, Selma Maria Bezerra
Oliveira, Edward José de
Nascimento, Frederico Crepaldi
Teixeira, Santuza Maria Ribeiro
Gazzinelli, Ricardo Tostes
Nagem, Ronaldo Alves Pinto
Fernandes, Ana Paula
Keywords: Diagnosis
Issue Date: 2019
Citation: SANTOS, A. R. R. dos et al. Evaluation of three recombinant proteins for the development of ELISA and immunochromatographic tests for visceral leishmaniasis serodiagnosis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-11, fev. 2019. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100310&lng=en&nrm=iso&tlng=en>. Acesso em: 7 mar. 2019.
Abstract: BACKGROUND Visceral Leishmaniasis (VL) is an infectious disease that is a significant cause of death among infants aged under 1 year and the elderly in Brazil. Serodiagnosis is a mainstay of VL elimination programs; however, it has significant limitations due to low accuracy. OBJECTIVE This study aimed to evaluate three recombinant Leishmania infantum proteins (rFc, rC9, and rA2) selected from previous proteomics and genomics analyses to develop enzyme-linked immunosorbent assay (ELISA) and immunochromatographic tests (ICT) for the serodiagnosis of human VL (HVL) and canine VL (CVL). METHODS A total of 186 human (70 L. infantum-infected symptomatic, 20 other disease-infected, and 96 healthy) and 185 canine (82 L. infantum-infected symptomatic, 27 L. infantum-infected asymptomatic, and 76 healthy) sera samples were used for antibody detection. FINDINGS Of the three proteins, rA2 (91.5% sensitivity and 87% specificity) and rC9 (95.7% sensitivity and 87.5% specificity) displayed the best performance in ELISA-HVL and ELISA-CVL, respectively. ICT-rA2 also displayed the best performance for HVL diagnosis (92.3% sensitivity and 88.0% specificity) and had high concordance with immunofluorescence antibody tests (IFAT), ELISA-rK39, IT-LEISH®, and ELISAEXT. ICT-rFc, ICT-rC9, and ICT-rA2 had sensitivities of 88.6%, 86.5%, and 87.0%, respectively, with specificity values of 84.0%, 92.0%, and 100%, respectively for CVL diagnosis. MAIN CONCLUSIONS The three antigens selected by us are promising candidates for VL diagnosis regardless of the test format, although the antigen combinations and test parameters may warrant further optimisation.
URI: http://www.repositorio.ufop.br/handle/123456789/11099
ISSN: 0074-0276
metadata.dc.rights.license: Todo o conteúdo do periódico Memórias do Instituto Oswaldo Cruz, exceto onde identificado, está licenciado sob uma licença Creative Commons 4.0 que permite copiar, distribuir e transmitir o trabalho em qualquer suporte ou formato desde que sejam citados o autor e o licenciante. Fonte: Memórias do Instituto Oswaldo Cruz <http://www.scielo.br/scielo.php/script_sci_serial/pid_0074-0276/lng_pt/nrm_iso>. Acesso em: 03 jan. 2017.
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