Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/11079
Title: Capillary zone electrophoresis method to assay tipranavir capsules and identification of oxidation product and organic impurity by quadrupole-time of flight mass spectrometry.
Authors: Lago, Matheus Wagner
Friedrich, Mariane Lago
Iop, Gabrielle Dineck
Souza, Thiago Belarmino de
Mello, Paola de Azevedo
Adams, Andréa Inês Horn
Keywords: Stability
Degradation products
High resolution mass spectrometry
Issue Date: 2018
Citation: LAGO, M. W. et al. Capillary zone electrophoresis method to assay tipranavir capsules and identification of oxidation product and organic impurity by quadrupole-time of flight mass spectrometry. Talanta, v. 181, p. 182-189, maio 2018. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0039914018300183?via%3Dihub>. Acesso em: 7 mar. 2019.
Abstract: Tipranavir (TPV) is one of the most recently developed protease inhibitors (PI) and it is specially recommended for treatment-experienced patients who are resistant to other PI drugs. In this work, a simple and friendly environmental CZE stability-indicating method to assay TPV capsules was developed and two TPV organic impurities were identified by high resolution mass spectrometry (HRMS). The optimized analytical conditions were: background electrolyte composed of sodium borate 50 mM, pH 9.0 and 5% of methanol; voltage + 28 kV; hydrodynamic injection of 5 s (100 mbar), detection wavelength 240 nm, at 25 °C. The separation was achieved in a fused silica capillary with 50 µm × 40 cm (inner diameter × effective length), using furosemide as internal standard. All the validation parameters were met and the method was specific, even in the presence of degradation products and impurities. Oxidation was indicated as the main degradation pathway among those evaluated in this study (acidic, alkaline, thermal, photolytic and oxidative) and it showed a second order degradation kinetic, under the conditions used in this study. The main oxidation product and an organic impurity detected in the standard were characterized by Q-TOF, and both of them correspond to oxidation products of TPV.
URI: http://www.repositorio.ufop.br/handle/123456789/11079
metadata.dc.identifier.uri2: https://www.sciencedirect.com/science/article/pii/S0039914018300183
ISSN: 0039-9140
Appears in Collections:DEFAR - Artigos publicados em periódicos

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